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Restriction Enzymes and DNA sequencing Exam Questions and Answers Already Passed

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Restriction Enzymes and DNA sequencing Exam Questions and Answers Already Passed restriction enzymes - Answers enzymes that can cut DNA at specific sequences What is another name for restriction enzymes? - Answers molecular scissors who discovered them and how? - Answers Arber and Lynn were st...

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  • October 21, 2024
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Restriction Enzymes and DNA sequencing Exam Questions and Answers Already Passed

restriction enzymes - Answers enzymes that can cut DNA at specific sequences

What is another name for restriction enzymes? - Answers molecular scissors

who discovered them and how? - Answers Arber and Lynn

were studying bacteria phages which are viruses that affected bacteria

discovered that there were special enzymes that would recognize phage and viral DNA and cut it up to
help protect the bacteria

restricting growth viruses

endonucleases - Answers in hot nucleic acid. cut at internal areas/location

exonucleases - Answers cut DNA at ends of the molecule

not restriction enzyme

Blunt end cutter - Answers when restriction enzyme cuts the DNA at same location in both strands

Staggered end cutter - Answers cut the DNA at 2 different places forming puzzle pieces

Restriction Digest - Answers cutting DNA with restriction enzymes

Linear DNA - Answers # of retraction fragments= # of cuts + 1

adds to the size or original DNA

draw figure on the gel

Circular DNA - Answers # of restriction fragments= # of cuts

O.R.I - Answers site were replication begins

reference point

Gene Cloning - Answers technique that allows you to generate multiple copies of a gene. all identical to
the original cell

when was the first human gene cloned? - Answers 1986

Clone - Answers Greek work for Klone meaning twig

group of cells that ate identical to the original cell

What are the ingredients of Cloning? There are 5 - Answers 1) gene of interest

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