Restriction Enzymes and DNA sequencing Exam Questions and Answers Already Passed
1 view 0 purchase
Course
Restriction Enzymes and DNA sequencing
Institution
Restriction Enzymes And DNA Sequencing
Restriction Enzymes and DNA sequencing Exam Questions and Answers Already Passed
restriction enzymes - Answers enzymes that can cut DNA at specific sequences
What is another name for restriction enzymes? - Answers molecular scissors
who discovered them and how? - Answers Arber and Lynn
were st...
Restriction Enzymes and DNA sequencing Exam Questions and Answers Already Passed
restriction enzymes - Answers enzymes that can cut DNA at specific sequences
What is another name for restriction enzymes? - Answers molecular scissors
who discovered them and how? - Answers Arber and Lynn
were studying bacteria phages which are viruses that affected bacteria
discovered that there were special enzymes that would recognize phage and viral DNA and cut it up to
help protect the bacteria
restricting growth viruses
endonucleases - Answers in hot nucleic acid. cut at internal areas/location
exonucleases - Answers cut DNA at ends of the molecule
not restriction enzyme
Blunt end cutter - Answers when restriction enzyme cuts the DNA at same location in both strands
Staggered end cutter - Answers cut the DNA at 2 different places forming puzzle pieces
Restriction Digest - Answers cutting DNA with restriction enzymes
Linear DNA - Answers # of retraction fragments= # of cuts + 1
adds to the size or original DNA
draw figure on the gel
Circular DNA - Answers # of restriction fragments= # of cuts
O.R.I - Answers site were replication begins
reference point
Gene Cloning - Answers technique that allows you to generate multiple copies of a gene. all identical to
the original cell
when was the first human gene cloned? - Answers 1986
Clone - Answers Greek work for Klone meaning twig
group of cells that ate identical to the original cell
What are the ingredients of Cloning? There are 5 - Answers 1) gene of interest
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller TutorJosh. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $7.99. You're not tied to anything after your purchase.