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DNA Cloning and Recombinant DNA Technology Exam Questions and Answers Already Passed

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Passed Short fragments - Answers Segments of DNA typically less than 100 bp. Long fragments - Answers DNA segments longer than 100 bp. Double strand DNA - Answers DNA consisting of two complementary strands. Reversible terminators - Answers Nucleotides that can be added and removed. PCR ampli...

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  • October 21, 2024
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  • 2024/2025
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  • DNA Cloning and Recombinant DNA Technology
  • DNA Cloning and Recombinant DNA Technology
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DNA Cloning and Recombinant DNA Technology Exam Questions and Answers Already Passed

Short fragments - Answers Segments of DNA typically less than 100 bp.

Long fragments - Answers DNA segments longer than 100 bp.

Double strand DNA - Answers DNA consisting of two complementary strands.

Reversible terminators - Answers Nucleotides that can be added and removed.

PCR amplification - Answers Technique to amplify DNA segments exponentially.

Single molecule sequencing - Answers Sequencing method that skips PCR amplification.

Nanopore sequencing - Answers Technique allowing real-time DNA sequencing.

Plasmid - Answers Circular DNA used as a vector in cloning.

Restriction endonucleases - Answers Enzymes that cut DNA at specific sequences.

Genomic libraries - Answers Collections of DNA fragments from an organism.

cDNA libraries - Answers Libraries representing mRNA from samples.

Reverse Transcriptase (RT) - Answers Enzyme converting RNA to complementary DNA.

Microarray analysis - Answers Technique measuring expression of multiple mRNAs.

In situ hybridization - Answers Technique visualizing specific RNA in tissues.

E. coli - Answers Common bacterium used for protein production.

Inducible lac promoter - Answers Promoter activated by lactose for gene expression.

Sticky ends - Answers Single-stranded ends of DNA after restriction cuts.

Transformation - Answers Process of introducing foreign DNA into cells.

DNA libraries - Answers Permanent collections of cloned DNA sequences.

Cloning - Answers Creating identical copies of DNA or cells.

Transcription factors - Answers Proteins regulating gene expression at transcription.

Histone code - Answers Regulatory code based on histone modifications.

Gene expression - Answers Process of converting DNA into functional products.

Recombinant DNA technology - Answers Techniques for combining DNA from different sources.

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