Recombinant DNA Technology Chapter 7 Review Questions with Complete Solutions Graded A+
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Recombinant DNA Technology Chapter 7
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Recombinant DNA Technology Chapter 7
Recombinant DNA Technology Chapter 7 Review Questions with Complete Solutions Graded A+
plasmid/vector - Answers small circular piece of DNA found in bacteria, often carry antibiotic resistance
restriction enzymes - Answers molecular scissors
restriction endonucleases - Answers act on DNA. reco...
Recombinant DNA Technology Chapter 7 Review Questions with Complete Solutions Graded A+
plasmid/vector - Answers small circular piece of DNA found in bacteria, often carry antibiotic resistance
restriction enzymes - Answers molecular scissors
restriction endonucleases - Answers act on DNA. recognize specific sequences and cut them. involved in
"restricting" growth of bacteriophage in certain bacteria
cloning - Answers fusing of 2 pieces of DNA together
recombinant DNA molecules - Answers constructed with DNA from different species. these can be found
in nature. (e.g., bacteriophage infecting bacteria and inserting its DNA into host)
methylation
adenine - Answers bacterial genome protects itself from EcoR1 by ______ at specific _____ residues
gel electrophoresis
agarose (or polyacrylamide) - Answers restriction fragments can be separated by __________ in
_______ gels
ethidium bromide - Answers the separated fragments of gel electrophoresis can be visualized by staining
with _________
DNA ligase
recombinant DNA - Answers the fragmented DNA and a vector are combined by ______ which
generates ______
plasmids
bacteriophages
viruses
artificial chromosomes - Answers cloning vectors can be:
MCS (multiple cloning site) - Answers multiple unique sites close together that can by cut by restriction
enzymes
ori - Answers origin of replication is abbreviated
Kan^r - Answers antibiotic resistance genes are abbreviated
rop - Answers gene that regulates replication for ~20 copies of the plasmid per cell (how many to
replicate for each cell)
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