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Steps of Recombinant DNA Technology Review Questions with Complete Solutions Rated 100%

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Steps of Recombinant DNA Technology Review Questions with Complete Solutions Rated 100% First Step - Answers Isolation of plasma DNa & DNA containing gene of interest 2nd Step - Answers Gene inserted into plasmid 3rd Step - Answers Plasmid put into bacterial cell 4th Step - Answers Cells clone...

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  • October 21, 2024
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  • Steps of Recombinant DNA Technology
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Steps of Recombinant DNA Technology Review Questions with Complete Solutions Rated 100%

First Step - Answers Isolation of plasma DNa & DNA containing gene of interest

2nd Step - Answers Gene inserted into plasmid

3rd Step - Answers Plasmid put into bacterial cell

4th Step - Answers Cells cloned with gene of interest

Fifth Step - Answers Identification of desired clone

Applications - Answers pest resistance, clean oil spills, when contain antifreeze, vaccine patterns

Central Dogma - Answers DNA, RNA, protein, trait

Uses for Molecular Biology - Answers Curing cancer, heart disease, Alzheimer's disease

Genetically manipulate corn for high yield & enhanced flavor

Genetically manipulate tomatoes for slower ripening

Identify criminals cases- murder or rape

DNA fingerprinting

Cytocrom C - Answers important electron carrier in respiratory pathaway, found in all aerobic
eukaryotes

Restriction endonucleases - Answers enzymes that have been purified from a different species of
bacteria and restriction enzyme

Restricting/digesting - Answers Cutting requires energy in form of ATP, involves physical cleaving of
chemical bonds,

specific recognition, sites where cuts occur and are palodromic

Plasmid - Answers Lab, Puc19 and E.Coli, relatively small extrocromisonal, circular molecule of DNA,
found in bacteria and yeast

Puc19 used for cloning because of size and DNA sequence

Agacrose gel - Answers Used to separate and sort fragments of DNA by size only, DNA fragments placed
in gel, electric current runs through matrix, fragments move at different rates depending on protein's
size and charge

Steps for cloning a gene and bacterial plasmid - Answers 1. Isolate DNA from two sources

2. Cut both DNA with some restriction enzyme

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