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Bio 140 Chapter 12 With Questions And 100% ALL CORRECT ANSWERS
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JACKLINEBio 140 Chapter 12 With Questions And 100% ALL CORRECT ANSWERS
Terms in this set (39)
deisconism for the genetic material
o Discovered dna copying mechanism
o The two strands of the parental duplex molecucle separate and each individual parental
watson and crick
strand serves as a model or template strand fothe synthesis of a daughter strand
o As each daughter strand is synthesized the order of the bases in the template strand
determines the order of the complementary bases added to the daughter strand
after replication the new dna duplex consists of one strand tat was originally part of the
semiconservative replication
parental duplex and one newly synthezised strand
proposes that the original dna duplex remain intact and the daughter dna duplex is completey
conservative replication
new
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, carried out an expereiment to determine how dna replicatioes
o The most beautiful experiement to determine how dna replciates
o Demonstrates the scientific method of hypothesis, prediction, and experimental tests
o Distinguished old from new dna strands by labeling them with nonradioactive isotopes of
nitrogen athat have diferent densities
o The normal form of nitoregen and a heavier form with an extra neutron
" Mathew s meseso and franklin w stahl o Found that dna replicates in a semiconservatively
o Predicted the results whne cells are allowed to undergo two rounds of replication in a
medium containing only light
o The heacvy strand and light trand each serve as templates for a new light daughter strand
o Halof of the dna molecules will have one heavy old strand and one light new strand and an
intermediate density and half of the dna moelcules will have one light strand and one light new
strand and a low density
allowed researchers to visusalize entire strands of eukarytotic dna and follow each strand
trhoguh replication in medium containting a floursecent neucleotide
o The chromosome was photgraphed at eataphase of the second round of mitosis after
" Labeling dna with fluorescet nucleotides
chromosome duplication but before the separation of the chromatids into the daughter celsls
o Results show that each chromosomal eukaryote contains a single dna molecule that runs
continuously all along its length
how do new dan strands grow by the addition of nucelotdies to the 3 prime end
Both daughter strands should grow in length by the addition of nucleotides near the sire
where the parental strands separate
o As the replication fork moves forward, both new strands would also grow in the direction of
replication fork movement
o The new daughter strands have opposite orientation so that near the replication fork the
replication fork
daughter strand in the bottom duplex terminates in a 5 prime hosphate
o The strand that ruminates terminates intthe 5 prime phosphate cannot grow in the direction
of the replication fork because the new dna strand can grow only by the addition of
successive nucleotides to the three prime end
o Dna always grows in a 5 to 3 prime direction
direction of dna polymerization only occurs in the 5 to 3 prime direction because of the chemistry of nucleic acid syntheissi
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