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MCB 2610 Lab Exam I Questions with complete solution 2024/2025
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MCB 2610 Lab Exam I Questions with complete solution 2024/2025 MCB 2610 Lab Exam I what is the quickest and most economical method of bacterial isolation? - correct answer streak plate method what is the purpose of aseptic technique? - correct answer maintain pure cultures what is th...

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  • October 17, 2024
  • 14
  • 2024/2025
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MCB 2610 Lab Exam I
what is the quickest and most economical method of bacterial isolation? -
correct answer ✔streak plate method


what is the purpose of aseptic technique? - correct answer ✔maintain pure
cultures


what is the purpose of a T-streak? - correct answer ✔isolate and maintain
single species from mixed populations


which objective lens should never be used with immersion oil? - correct
answer ✔40x


procedure for making smears - correct answer ✔1. use a wax pencil to draw
a circle on the slide
2. take a loop of distilled water and smear it on the slide
3. take a very small loop of culture
4. smear it onto distilled water
5. allow to dry then heat fix, smear side up 3x


what type of stain is used for simple staining? - correct answer ✔simple
crystal violet stain


if the chromophore is a positive ion, the stain is considered a(n)
______________________ stain - correct answer ✔basic

, if the chromophore is a negative ion, the stain is considered a(n)
__________________ stain - correct answer ✔acidic


what is the mordant used in the gram stain and what does it do? - correct
answer ✔iodine - it makes the color permanent


bacteria that retain the Gram stain are classified as Gram _____________ -
correct answer ✔positive


what do Gram positive bacteria have that allows them to retain the primary
stain? - correct answer ✔thick wall of peptidoglycan


why are Gram negative bacteria easy to decolorize? - correct answer ✔they
have an outer membrane and a thinner layer of peptidoglycan


do Gram negative stains retain the counterstain (safranin)? - correct answer
✔yes


why is the spore stain used with bacteria such as Bacillus and Clostridium? -
correct answer ✔they develop an endospore in response to certain
environmental conditions such as depletion of nutrients


T/F heat used during staining causes sporulation - correct answer ✔FALSE


Procedure for Gram Stain - correct answer ✔1. prepare a smear of bacteria
2. flood the surface with crystal violet
3. wait one minute and then pour off the excess stain
4. wash gently with water
5. flood the stain with Gram's iodine

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