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MCB 2610 Lab Exam 1 Questions with complete solution 2024/2025
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MCB 2610 Lab Exam 1 Questions with complete solution 2024/2025 MCB 2610 Lab Exam 1 T streak method - correct answer ussed to isolate colonies 1. steralize loop by placing in flame 2. dip into media 3. spread on zig zap in 1 4. steralize then drag from 1 to 2 5. steralize than drag from 2...

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  • October 17, 2024
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  • 2024/2025
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MCB 2610 Lab Exam 1
T streak method - correct answer ✔ussed to isolate colonies
1. steralize loop by placing in flame
2. dip into media
3. spread on zig zap in 1
4. steralize then drag from 1 to 2
5. steralize than drag from 2 to 3


colony - correct answer ✔community of genetically identical cells that can be
seen without a microscope


liquid vs solid media - correct answer ✔liquid= grow large amounts of
bacteria for testing ex. nutrient broth


solid= examine morphology, isolation ex. nutrient agar, solid polysacharide


complex vs defined media - correct answer ✔complex= don't know whats in
it, yeast extract, TSB broth less nutrients, general purpose media
defined= know whats in it ex. synthetic, has more nutrients
not all bacteria can be cultured


selective media vs differential - correct answer ✔suppress unwanted
microbes and encourage desired microbes ex. sf
differential= allows bacteria to be differentiated from others by color change
ex. macconkeys

, SF medium - correct answer ✔selective= e faecalis
grows E. faecalis= intestinal bacterium
contains sodium azide
inhibits gram negtive growth
ferments glucose to only grow gram +
control= macconkeys bc gram + can't grow


MacConkey Agar - correct answer ✔SELECTIVE and DIFFERENTIAL
selective= gram negative organism by bile salts, coliforms
differential = lactose (lactose positive organism will look pink negative will be
clear)
control= na glucose plate bc everything will grow on it


hydrolytic enzymes - correct answer ✔break down proteins and certain
carbohydrates.
amylase= breaks down starch
casease (casinase)= breaks down milk protein casein
gelatinase= breaks down gelatin


amylase test - correct answer ✔plate contains starch breaks down starch,
requires iodine which stains starch
positive= amber color
negative= black color


caseinase test - correct answer ✔medium contains casein
pos= clearing
negative= no clearing around growth'

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