BIOD 171 Portage Learning Exam 3 Questions With Verified Answers
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Course
BIOD 171
Institution
BIOD 171
BIOD 171 Portage Learning Exam 3
Questions With Verified Answers
Define the measurements micrometer and nanometer. - answerA micrometer is one-millionth
of a meter and a nanometer is one-billionth of a meter.
What are the two critical factors that influence your ability to see an object? - answ...
BIOD 171 Portage Learning Exam 3
Questions With Verified Answers
Define the measurements micrometer and nanometer. - answer✔A micrometer is one-millionth
of a meter and a nanometer is one-billionth of a meter.
What are the two critical factors that influence your ability to see an object? - answer✔The
resolution and the contrast. Resolution is the distance between two objects where you can still
see them as separate. Contrast is the difference in light between two objects.
If you wish to increase the amount of light going into a microscope, what part would you adjust?
- answer✔The iris diaphragm.
As light passes through a microscope, what is the last piece that light passes before reaching your
eyes? - answer✔Once light passes through the sample and the objective lens, it passes through
the OCULAR LENS.
How is the total magnification of an object calculated? - answer✔It is calculated by multiplying
the power of the objective and the power of the eyepiece.
What is one limitation of fixing your sample? - answer✔Fixing your sample requires you to
irreversibly kill your sample.
This can make it impossible to determine the cell movement. You could distort the shape and
structure.
Phase-contrast microscopy provides what benefits to imaging? - answer✔Phase-contrast
microscopy provides detailed images without staining.
What is the distinguishing feature of dark field microscopy? - answer✔Dark field microscopy
reflects light off of the specimen at an angle..
Unlike brightfieqld microscope, fluorescence microscopes illuminate samples through what
spectrum? - answer✔The Ultraviolet Spectrum.
What is the primary difference between TEM and SEM? - answer✔The electron passes through
the sample during TEM where as the electron is reflected off the sample during SEM.
Gram staining is based on what basic principle? - answer✔With the basic observation that
different types of bacteria react differently to various dyes.
What is a key determinant in bacteria being Gram-positive? - answer✔Gram-positive have a
thick peptidoglycan layer. Uses crystal violet and iodine. The resulting mixture is a purple
coloration of the cell.
What is the purpose of heat fixing a sample? - answer✔Heat fixing makes sure the samples are
tightly adhere to the glass slide.
What is the primary purpose of a wet mount? - answer✔Wet mounts are often perform to
visualize live cells and to see the motility and behavior of an organism.
The acid-fast stain is most often used to identify what specific microorganism? -
answer✔Mycobacterium tuberculosis is the most common for an acid-fast stain.
What is the total magnification of a sample imaged with a 60x objective and a 10x eyepiece? -
answer✔60 x 100 = 600x magnification
True or False: A cell that is adherent, flat, and unstained is easily identified using bright field
microscopy. - answer✔False
Flat cells are almost invisible due to the limits of resolution and contrast
Which of the following could be seen clearly by the unaided eye? - answer✔Protozoa with
diameter of 150um and skin cell with diameter of 1500um
Label the following microscope components by matching it with the components provided. -
answer✔1. Eyepiece
2. Neck
3. Fine adjustment knob
4. Objective
5. Stage
6. Base
This type of microscope is best suited for visualizing GFP, RFP, and YFP proteins -
answer✔Flourescence
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