NWHSU Microbiology - Lab Final Exam Questions Verified Answers Current Update (A+ Pass)
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NWHSU Microbiology - Lab Final Exam Questions Verified Answers Current Update (A+ Pass)
Define titer - Answers - The highest dilution of a sample that tests positive for an antibody.
Which immunoglobulin appears first when someone has an infection? - Answers - IgM
Define Prozone. - Answers...
NWHSU Microbiology - Lab Final Exam
Questions Verified Answers Current
Update (A+ Pass)
Define titer - Answers -✔✔ The highest dilution of a sample that tests positive for an
antibody.
Which immunoglobulin appears first when someone has an infection? - Answers -✔✔
IgM
Define Prozone. - Answers -✔✔ During antibody precipitation tests, its the period of
time when antibody concentrations are high and no precipitates are formed, leading to
false negatives.
How is a positive Western blot for HIV determined? - Answers -✔✔ There will be two or
more dark bands.
what are the reagents in gram staining? - Answers -✔✔ Crystal violet, Grams iodine,
acetone-alcohol, safranin.
In gram staining, what does crystal violet do? - Answers -✔✔ stains all gram + and -
bacteria.
In gram staining, what does grams iodine do? - Answers -✔✔ its a mordant that forms
violet-iodine complex that binds more.
In gram staining, what does acetone-alcohol do? - Answers -✔✔ It decolorizes gram -
bacteria. But only if not done for too long.
In gram staining, what does safranin do? - Answers -✔✔ counterstains gram - bacteria.
What color are gram positive bacteria? - Answers -✔✔ Purple
What color are gram negative bacteria? - Answers -✔✔ pink
How do gram stains and wet mounts vary? - Answers -✔✔ Gram can determine gram
+/-. Wet mounts can tell motility.
How are gram stains and wet mounts similar? - Answers -✔✔ They both can see
arrangement, size, and shape.
, What is the KOH test to differentiate gram positive from gram negative bacteria? -
Answers -✔✔ Mix KOH with a colony for 20 seconds. Stringy=negative. Not
stringy=positive.
What are 3 spore forming bacteria that cause disease? - Answers -✔✔ Bacillus
Anthracis, Clostridium tetani, Clostridium botulinum.
What bacteria causes anthrax? - Answers -✔✔ Bacillus Anthracis
WHat bacteria causes Lock jaw - Answers -✔✔ Clostridium tetani
What bacteria causes botulism - Answers -✔✔ Clostridium botulinum
Define alpha hemolysis and what does it look like? - Answers -✔✔ Bacteria that can
partiatlly break down heme pigment (green halo)
Define beta hemolysis and what does it look like? - Answers -✔✔ bacteria that can
break down heme pigment fully. (clear zone)
Define gamma hemolysis and what does it look like? - Answers -✔✔ bacteria that show
no change to a blood agar plate.
Which hemolysis is a lab looking for in diagnosing strept throat? - Answers -✔✔ beta
hemolysis
What is the importance of a capsule? - Answers -✔✔ It protects against microphages
in lungs.
What stain shows a capsule? - Answers -✔✔ gin stain.
what is the name of the acid-fast stain and what is it used for? - Answers -✔✔ Ziehl-
Neelson procedure uses carbol fushsin. It detects bacteria with cell walls rich in lipids
and waxes. LIke Mycobacterium and Nocardia. Leprosy, TB,
Define selective media? - Answers -✔✔ Isolates/cultivates for specific bacteria
Define differential media? - Answers -✔✔ Shows chemical reactions of bacteria
Is MacConkeys selective or Differential? - Answers -✔✔ Both
Is blood agar selective or differential? - Answers -✔✔ Differential
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