MCB 2050 Midterm Exam Questions and Answers 100% Solved
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Course
MCB 2050
Institution
MCB 2050
MCB 2050 Midterm Exam Questions and
Answers 100% Solved
1) What are restriction endonucleases?
2) What do they do (or cut) specificically?
3) What happens to the ends of DNA cut by restriction enzymes? - 1)
enzymes that cut at specific sequences (restriction sites) within DNA
molecules
2) th...
-small, up to about 10kb (for easy manipulation; maximum insert size
usually approx 15kilobase pairs)
3) -an origin of DNA replication (for amplification in bacterial cells)
-a selectable marker: antibiotic resistance gene
-at least one unique RE cleavage site outside of the origin and resistance
gene (many have multiple cloning sites, MCS)
What are the characteristics of the pBluescript cloning vector? - ✔✔-
bacterial origin of replication (to allow plasmid replication)
-multiple cloning site (MCS) to allow insertion of foreign DNA
-antibiotic resistance gene for selection of bacteria transformed with the
plasmid
-selectable marker (lacZ) to differentiate cells with plasmids containing
inserted foreign DNA from those without (blue/white screening)
-lac promoter to express mRNA (small (3kb) to allow for large insertions)
-all RE sites in the MCS are unique sites in the plasmid
1) What does the bluescript plasmid express?
2) Where is the MCS located?
3) What does insertion of foreign DNA into the MCS do? What is this
called? - ✔✔1) the E.coli lacZ gene (encodes beta-galactosidase)
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