Exam (elaborations)
Immunohematology Exam 2 (ABO Discrepancies, HTLA, Mixed Field, Transfusions RXNS, AIHA, HDFN )
- Course
- Institution
Immunohematology Exam 2 (ABO Discrepancies, HTLA, Mixed Field, Transfusions RXNS, AIHA, HDFN ) ...
[Show more]
Content preview
Immunohematology Exam 2 (ABO Discrepancies, HTLA, Mixed Field,Transfusions RXNS, AIHA, HDFN ) 2024-2025
HTLA characterictics
- weak reactions when undiluted (low avidity)
- react at high dilutions 1:2048 (high titer)
- weak at AHG phase
- variable strength of reactivity
- nonreactive with autologous cells
- most are high prevalence antigens
HTLA non reactive with ficin treated RBCs
-Anti-Ch, -Rg (Chido Rogers)
•neutralizable: Anti-JMH
Anti-Ch or -Rg
- absorb with autologous RBCs coated with C4d
- use plasma suspended cells
- neutralization
HTLA reactive with ficin treated RBCs
-Anti-Kna, -McCa, -Sla, -Yka. (Knops)
-Anti-Hy, -Gya (Dombrock)
-Anti-Csa (COST collection)
-Anti-Sda
,Detect HTLA antibodies
-RT testing
-fresh panel and donor RBCs
-albumin
-60 min incubation at 37
-AHG phase
-inhibition study: pooled plasma from antigen positive individuals or adsorb with C4
coated cells (anti -Ch, -Rg)
Clinical significance of HTLA antibodies
- most are clinically insignificant
- except anti-Hy and anti-Gya (mild HDN, shortened survival of transfused antigen
positive cells)
- associated with autoimmune disease
ABO Discrepancies Flowchart
Missing/weak forward reactions
A or B Subgroup
Disease States
BGSS
Resolution for Missing/weak in the forward
Wash red cell suspension and incubate for 30 minutes at room temperature with the
addition of anti-A, -B.
, Enzyme treat patient's red cells in order to enhance the reaction.
- Use O red cells as control
Saliva testing
- Only if patient is a secretor
Adsorption/Elution
Describe the use of Adsorptions/Elutions in resolving missing reactions in the forward.
Adsorption/Elution
1) Patient's cells incubated at 4C with appropriate antisera
2) Take the cells with attached antibodies and do an eluate to take those antibodies off
3) Take that eluate and test it against A, B, and O cells
Additional in the forward
Acquired B or B(A) phenotype
Polyagglutination
Rouleaux
Cold autoantibodies
Contamination in reagent/Reagent dye
Bone Marrow Transplant
How does the (B)A phenotype give an extra reaction in the forward? How is it resolved?
High levels of B galactosyltransferase transfers small amounts of the A sugar to the H
antigen
- Detected by murine monoclonal antibody in reagent (MHO4)
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller Chrisyuis. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $11.99. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
75759 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now