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UNE BIOCHEM MIDTERM EXAM
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UNE BIOCHEM MIDTERM EXAMDephosphorylation by a phosphatase requires all of the following except:
-ATP
-Water
-Mg2+ – ATP
Explain the roles of protein kinases, protein phosphatases, regulatory, hormonal,
and second messengers in regulating metabolic processes via covalent and
allosteric interactions.
During competitive inhibition, Km is_________.
-Increased
-Decreased
-Unchanged
Unsaturated - ANSWER Increased
Describe the use of linear forms of the Michaelis-Menten equation to
estimate Km and Vmax.
Consider the effects of increasing substrate concentrations on the kinetics of
simple competitive and noncompetitive inhibition.
The Michaelis-Menten equation relates velocity (vi) to the Michaelis constant
(Km).
, -Inversely
-Directly
-Equally
-Proportionally, ANSWER Inversely.
Describe the use of linear forms of the Michaelis-Menten equation to estimate
Km and Vmax.
A novel enzyme is identified that rises in activity when thiamine is bound at a
position other than the active site. This form of enzyme regulation is classified
as which of the following.
Transcriptional activation
-Covalent modification.
-Feedback inhibition
-Answer: Allosteric activation.
Describe two methods for constructing regulatory networks in cells.
Which of the following is correct in terms of enzyme kinetics if a mutation
occurs within an enzyme's active site, resulting in an increase in Km?
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