BIOSAFETY AND BIOSECURITY Test 4| Questions
with Answers Verified 100% Correct
How are infectious agents classified by NIH? - ✔✔into ascending risk groups from 1-4
-higher # indicates higher risk of hazardous characteristics that may cause human disease
How are infectious agents classified by WHO? - ✔✔based on degree of Dz hazard to humans
and animals, natural route of the Dz's transmission, preventative measures, and effective
treatments
WHO - ✔✔World Health Organization
Genetically Modified Agent Hazards - ✔✔possibility that the genetic modification could
increase an agent's pathogenicity or affect its susceptibility to effective tx
Dangers of Aerosols - ✔✔typically undetected and are extremely pervasive
-those containing infectious particles are hazardous even when inhalation may not be a natural
route of transmission of disease
Most important element of containment - ✔✔strict adherence to standard microbiological
practices and techniques
What are examples of primary barriers? - ✔✔Safety Equipment and PPE:
-Biological Safety Cabinet
-gloves, safety glasses, gowns, shoe covers
• Class I and II BSC - ✔✔protection to both the lab personnel and environment when used
with good microbiological practices
, Class II & III BSC - ✔✔protects research materials from airborne contaminants by using a
HEPA filter
BSC class 1 - ✔✔Personnel and Environmental Protection only!
-unfiltered air from the room, so no protection to what is inside the cabinet
BSC class 2 - ✔✔Draws air into and out of the cabinet and filters it both ways
-Personnel, Environmental, and Product protection
BSC Class 3 - ✔✔glove boxes
-Total Containment: Personnel do not come into contact with the items inside the cabinet
ABSL - ✔✔Animal Biosafety Levels
-Guidelines for the use of experimentally infected animals & for the maintenance of laboratory
animals that may naturally harbor zoonotic infectious agents
Facilities must be in compliance with what for employee safety? - ✔✔US Department of
Labor (DOL)
-OSHA regulations
-pt confidentiality laws
-the Americans with Disabilities Act (ADA) of 1990
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