BIOSAFETY AND BIOSECURITY Test 3| Questions
with Answers Verified 100% Correct
Secondary barriers for BSL include - ✔✔BSL-1 barriers plus autoclave for glassware
biosafety sign posted outside lab working with infectious agents must include what? -
✔✔Lab's biosafety level
Infectious agents under study
Contact information for responsible person and 2 emergency contacts
Needles & Sharps Precautions for BSL 2 include - ✔✔DON'T
Break, bend, re-sheath or re-use syringes or needles
DO
Use sharps containers
DON'T
Touch broken glass with hands
DO
Use plasticware
Safety practices for BSL 2 include sharps as well as _____ proof containers - ✔✔leak proof
DOUBLE containers
, With BSL special practices include a supervisor that is a __________ with increased
responsibilities that ______ Access if Immunocompromised
Restricts Access to ______ - ✔✔competent scientist that limits access
Immunized
BSL 2 primary barriers - ✔✔BSL-1 PLUS:
Use biosafety cabinets (class II) for work with infectious agents involving:
Aerosols and splashes
Large volumes
High concentrations
Class II Biosafety Cabinets
Layout of equipment - ✔✔(work clean to dirty: L to R)
Which level has agents with potential for respiratory transmission and may cause serious and
potentially lethal infection - ✔✔BSL 3
Primary barriers for BSL 3 - ✔✔Similar to BSL-2 personal protective equipment
Respiratory equipment if risk of infection through inhalation
Secondary barriers for BSL 3 - ✔✔All BSL-2 barriers
Corridors separated from direct access to lab
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