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Exam (elaborations)

LCMS EXAM 2024 QUESTIONS & ANSWERS SOLVED 100% CORRECT!!

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  • Course
  • LCMS
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  • LCMS

LCMS EXAM 2024 QUESTIONS & ANSWERS SOLVED 100% CORRECT!!

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  • October 9, 2024
  • 15
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • what is hplc used for
  • LCMS
  • LCMS
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EvaTee
LCMS EXAM 2024
QUESTIONS &
ANSWERS SOLVED
100% CORRECT!!
LCMS

Evatee 10/9/24 LCMS

, LCMS EXAM 2024 QUESTIONS & ANSWERS
SOLVED 100% CORRECT!!


What is HPLC used for? Answer - hydrophilic & nonvolatile components in
mixtures


What is HPLC? Answer - uses high pressure to force eluent through a column
packed with small particles that provide separations of pg to ug of analytes.


What are advantages of HPLC? Answer - high accuracy
high sensitivity
automated systems from sample prep to report generation
high speed
high resolution
can do prep work


What are disadvantages of HPLC? Answer - expensive instrumentation
experience required
no universal detector yet -> UV-vis, fluorescence, refractive index?
needs extra technique for confirmation of identity
expensive supplies


What are typical nonvolatile compounds? Answer - pharmaceuticals such as
aspirin, ibuprofen
proteins such as egg white or blood protein

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