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Lesson 18 (FINAL) Biology 1105 with 100- correct answers(graded A+).
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Lesson 18 (FINAL) Biology 1105 with 100- correct answers(graded A+).
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Lesson 18 (FINAL) Biology 1105with 100% correct
answers(graded A+)
Distinguish between selective breeding and recombinant DNA
engineering as approaches to manipulate an organism's genome.
What are advantages and disadvantages to each approach ? -
answer Selective Breeding: uses controlled mating to develop an
organism w/ the desired characteristics
> can take many generations to achieve desired results
> may result in selection of undesirable/ harmful traits along w/
desirable ones
Recombinant DNA technologies: involves the manipulation of DNA
using enzymatic reactions
> DNA sequences from multiple sources can be "recombined" &
inserted into an organism
> can introduce traits what are not native to the organism
> much faster method of trait introduction that selective breeding
What is a restriction enzyme and what is the normal function of
these proteins in bacteria? What are sticky ends? - answer
Describe the three steps of a PCR cycle and the purpose of each.
How is PCR like DNA replication in the cell and how is it different? -
answer PCR steps:
1. Denaturing: DNA is denatured into single strands(95C)
2. Annealing: Primers anneal to DNA ( 50-60 C)
3. Synthesis/Extension: Taq DNA polymerase (72C)
PCR is like DNA Replication:
> DNA is unwound
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