A pediatrician contacted the laboratory to ask which test or tests would be
most useful in the case of a child suspected of having complications
following Strep throat. The technologist asked this physician some questions
about what she was trying to determine and found that the pediatrician
wanted the test or tests most likely not to miss potential antibodies produced
against streptococcal antigens. Which of the following should the
technologist recommend?
a. Streptozyme test followed, if positive, by ASO
b. ASO test followed, if positive, by Streptozyme
c. Culture followed, if positive, by Rapid Strep cassette testing
d. Rapid Strep cassette testing followed, if positive, by culture - ✔️
✔️a.
Streptozyme test followed, if positive, by ASO
A patient tests negative for rheumatoid factor. The patient does have
symptoms consistent with rheumatoid arthritis. The physician should
a. Do further testing because the RF test is negative in approximately 25% of
patients who have the disease.
b. Change the diagnosis to something other than RA because the RF test is
positive in 100% of patients with the disease.
c. Ask the laboratory to repeat the test on a second sample immediately
because the only way to diagnose RA is through the use of the RF.
,d. Ask the laboratory to repeat the test on a plasma sample because the test
should NOT be done on serum. - ✔️ ✔️ a. Do further testing because the RF
test is negative in approximately 25% of patients who have the disease.
Clostridium difficile is the leading cause of
a. the common cold.
b. adult respiratory syndrome.
c. food poisoning.
d. nosocomial diarrhea. - ✔️
✔️d. nosocomial diarrhea.
Which of the following best describes what is occurring in the slide latex
agglutination test for rheumatoid factor when the test is positive?
a. Patient produced rheumatoid factor binds to the IgG bound to the carrier
particles producing agglutination.
b. Reagent IgG binds to the patient's red cells if they have produced
rheumatoid factor inhibiting agglutination.
c. Patient produced rheumatoid factor binds to reagent antigens bound to the
slide and then latex particles adhere and show color if RF is present.
d. Murine IgM binds to the patient IgG that has bound to the latex particles in
the test, producing agglutination. - ✔️
✔️ a. Patient produced rheumatoid
factor binds to the IgG bound to the carrier particles producing agglutination.
The type of fluorescent immunoassay that is based on the change in
polarization of fluorescent light emitted from a labeled molecule when it is
bound by antibody and is a competitive assay in which unlabeled antigen in
the patient sample competes with labeled antigen for a limited number of
antibody binding sites is
a. FPIA.
, b. RIBA.
c. chemiluminescence.
d. FANA. - ✔️
✔a️. FPIA.
Which technique is used to detect antigen-antibody reactions that can be
seen with a fluorescent microscope?
a. Enzyme Immunoassay (EIA)
b. Fluorescent Antibody (FA)
c. Direct Immunofluorescent Assay (DFA)
d. Enzyme-linked immunosorbent assay (ELISA) - ✔️
✔c️. Direct
Immunofluorescent Assay (DFA)
The diseases known as mono or kissing disease, actually infectious
mononucleosis, is caused by
a. Hepatitis D virus.
b. Pseudomonas aeruginosis
c. the Epstein-Barr virus.
d. the parainfluenza virus. - ✔️
✔c️. the Epstein-Barr virus.
One of the most commonly used tests for antinuclear antibodies is
a. indirect immunofluorescence.
b. equine serum agglutination.
c. a skin test.
d. reverse polarization. - ✔️
✔a️. indirect immunofluorescence.
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