Janeways Immunobiology 9th Edition by Kenneth Murphy; Casey Weaver |
Janeways Immunobiology 9th Edition by Kenneth Murphy; Casey Weaver |
Janeways Immunobiology 9th Edition by Kenneth Murphy; Casey Weaver |
All for this textbook (19)
Written for
Vrije Universiteit Amsterdam (VU)
Oncology
Tumor Immunology
All documents for this subject (6)
Seller
Follow
oddsters
Content preview
Module 2: Tumor Immunology (Week 3)
LECTURE 21: TOOLS IN TUMOR IMMUNOLOGY/SINGLE CELL TECH.
Cytometry examines cell characteristics: size, count, morphology, phase/cycle, DNA content & presence of
specific protein HD cytometry: addressing molecule expression at single cell level (highly specific); allows
discovery of rare cells/molecules
, Module 2: Tumor Immunology (Week 3)
Cytometry allows examination of cells as
single cell/individual: behavioral change
upon certain stimulation (e.g. oncogenic
mutation) & response of each cell to certain
drug
Fundamental principle of cytometry (conventional)
Determination of normal & abnormal
functions of the cells
Single cell suspension goes through the
sheath laser dispersion (light source)
collected by optics system (prism, mirror,
filter) electronic amplification
analysis
Examining the morphology & functional
characteristics from tissue biopsy
Cells extracted from tissue sample is loaded with
antigen put through the suspension tube
analysis of morphology & function at the same time
(TOF-MS)
Different wavelength/light source to examine
different function composite/concomitant
analysis (e.g. cell status, immune cell types, regulatory
functions/Treg, IDO, etc, stromal conditions)
, Module 2: Tumor Immunology (Week 3)
HD Cytometry (less noise d/t reduced scatter)
Metal-elements based: mass cytometry (using rare natural elements)
Signal detection affected by: antigen level, sensitivity of sensor, background signals & cross-talk between
abundance sensitivity, isotope purity, and metal ion oxidation
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller oddsters. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $4.28. You're not tied to anything after your purchase.
