Amino acid in polypeptides are covalently bound by what type of bond?
a) Peptide
b) protein
c) ester
d) phosphodiesterpeptide
An alpha helix is what kind of protein structure?
a) secondary
b) primary
c) tertiary
d) quaternarysecondary
If you disrupt all hydrogen bonds in a protein, what l...
MIP 616 Exam 1 Multiple Choice, MIP 616 test 1,
MIP616 Exam 1 study
Questions and Answers
Amino acid in polypeptides are covalently bound by what type of bond?
a) Peptide
b) protein
c) ester
d) phosphodiester✅✅peptide
An alpha helix is what kind of protein structure?
a) secondary
b) primary
c) tertiary
d) quaternary✅✅secondary
If you disrupt all hydrogen bonds in a protein, what level will preserved?
a) secondary
b) primary
c) tertiary
d) quaternary✅✅primary
Monomeric proteins do not contain a...
a) secondary
b) primary
c) tertiary
d) quaternary✅✅quaternary
Which is not part of a zinc finger motif?
a) zinc ion
b) proline residue
c) cysteine residue
d) histidine residue✅✅proline residue
a small molecule that binds directly to the active site of an enzyme and disrupts its catalytic
reaction is called:
a) an allosteric inhibitor
b) a competitive inhibitor
c) a noncompetitive inhibitor
d) RNAi✅✅a competitive inhibitor
Which statement about enzymes is TRUE?
a) The catalytic site is responsible for substrate specificity.
,b) Reactions catalyzed by enzymes give the products more free energy than reactions that occur
spontaneously.
c) The rate of an enzymatic reaction is always proportional to the concentration of the substrate
d) Enzymes increase reaction rates by lowering the activation energy needed to reach the
transition state?✅✅Enzymes increase reaction rates by lowering the activation energy needed to
reach the transition state?
Which modification marks a protein for degradation in proteasomes?
a) Phosphorylation
b) Ubiquitinoylation
c) acetylation
d) glycosylation✅✅ubiquitinoylation
Kinases activates or inactivate many proteins by doing what to them?
a) methylate
b) phosphorylate
c) acetylate
d) digest✅✅phosphorylate
The conversation if inactive chymoptrypsinogen to active chymotryosin is an example of?
a) proteolytic activation
b) positive cooperativity
c) allostery
d) Ligand induced activation✅✅proteolytic activation
Which amino acid can be phosphorylated?
a) lysine
b) serine
c) glycine
d) proline✅✅serine
G proteins act as...
a) a catch
b) a branch
c) a switch
d) a notch✅✅a switch
what do g proteins do to their bound nucleotide triphosphate?
a) nebulize it
b) hydrolyze it
c) customize it
d) homogenize it✅✅hydrolyze it
What kinds of proteins accelerate nucleotide hydrolysis by G proteins?
a) guanine nucleotide exchange factor
,b) GTPase accelerating protein
c) nuclear localization signal proteins
d) nucleoproins✅✅GTPase accelerating protein
In two-dimensional gel electrophoresis, proteins are first resolved by... and then by...
a) isoelectric focusing; SDS-PAGE
b) SDA-PAGE; affinity chromatography
c) SDS-PAGE; ion exchange
d) isoelectric focusing; gel filtration✅✅Isoelectric focusing; SDS-PAGE
Gel filtration chromatography separates proteins on the basis of their:
a) charge.
b) mass.
c) affinity for a ligand.
d) shape.✅✅mass
Which detergent is used to disrupt protein structure for electrophoretic analysis on
polyacrylamide gels?
a) Tide
b) Triton X-100
c) Nonidet P40 (NP40)
d) Sodium dodecylsulfate (SDS)✅✅sodium dodecylsulfate (SDS)
Tissue is treated to break open each cell's membrane and release the contents, and then subjected
to differential centrifugation. Which of the following is TRUE after centrifugation?
a) Depending on centrifugation speed, proteasomes are more likely to be in the supernatant than
chaperones.
b) Proteins of similar density will be found in the same fraction (either pellet or supernatant).
c) The pellet contains the least dense material.
d) The pellet will contain a purified protein for further analysis.✅✅Proteins of similar density
will be found in the same fraction (either pellet or supernatant).
Proteomics allows researchers to:
a) examine where a protein is located within the cytosol
of a cell.
b) compare thousands of samples from different people in the same experiment.
c) examine which proteins differ in abundance between a normal sample and a disease sample.
d) use antibodies to label specific proteins on an SDS-PAGE gel.✅✅examine which proteins
differ in abundance between a normal sample and a disease sample.
What factors necessary for growth of animal cells
in culture are provided by serum?
a) amino acids
b) precursors of DNA synthesis
c) growth factors
, d) vitamins✅✅growth factors
Which amino acid can be phosphorylated?
a) lysine
b) threonine
c) glycine
d) proline✅✅threonine
Which is the BEST technique/approach to localize
catalase in peroxisomes?
a) a catalase monoclonal antibody and transmission
electron microscopy
b) platinum or gold and scanning electron microscopy
c) FRAP and FRET
d) All of the answers are correct.✅✅a catalase monoclonal antibody and transmission
Which of these amino acids can be ubiquitinylated?
a) lysine
b) threonine
c) glycine
d) proline✅✅threonine
The purpose of treating tissue from an embryonic chick with trypsin and EDTA when generating
a primary cell culture is to:
a) break down proteins present in the serum so the cells can use the amino acids for energy.
b) cleave cell surface proteins so you can grow the chick cells as a suspension culture.
c) digest protein-protein interactions that hold cells together in a tissue.
d) prevent cell senescence.✅✅digest protein-protein interactions that hold cells together in a
tissue
Which of these epithelial cell surfaces is involved in secreting proteins out of the cell?
a) apical
b) basal
c) lateral
d) basolateral✅✅apical
Which type of drug would reduce the amount of antibody produced by hybridomas?
a) proteasome inhibitors
b) protease inhibitors
c) transcription inhibitors
d) antibiotics✅✅transcription inhibitors
The phenomenon in which a chemical absorbs light at one wavelength and emits it at a specific
and longer wavelength is called:
a) differential interference contrast.
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