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Bio 210 Enzyme Lab Report

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This is a comprehensive and detailed lab report on; identifying the roles of enzymes as catalysts and identifying the substrate and the product for Bio 210. *Essential Study Material!!

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  • October 1, 2024
  • 4
  • 2021/2022
  • Other
  • Unknown

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Anissa Zibo

Identifying the role of Enzymes as Catalysts and Identifying the Substrate and Product of

an Enzyme

Introduction: Enzymes are special types of proteins that are made up of different amino acids in

a long chain. The specific function of an enzymes is determined by their sequence of amino

acids. Enzymes are substances produced by living organisms that act as catalysts to speed up

chemical reactions. Enzymes also reduce the activation energy needed in a reaction by binding to

reactants. The reactants of an enzyme reaction are called substrates. Substrates are what bind to

active sites through hydrogen bonds.

Though enzymes are useful in speeding up reactions, there are a number of factors that

affect how an enzyme will work. Temperature is one of them. The higher the temperature the

faster the enzyme will work, but if the temperature becomes too hot the enzyme will denature

and breakdown. PH levels can also affect the ability for an enzyme to do its job, too high or low

of a pH can ultimately stop a reaction.

Hypothesis: The standard curve of maltose will correlate maltose concentration with absorbance

and will determine the amount of maltose produced as starch is digested with amylase.

Materials: The materials we used to prepare the starch was conical flask, beakers, graduated

cylinders, pipettes, glass plate, hot plate, thermometer, water bath, potato starch powder, iodine,

sodium phosphate and buffer. For the second, we used beakers, graduated cylinder, test tubes,

test tube rack, hot plate, spectrometer, maltose, cuvettes, pipettes, pipettes bulbs, kimwipes,

distilled water, dinitrosalicylic acid and a marking pencil. The same materials were used in

experiment 3.

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