BIOL 2420 Lab Practical 1 Test Questions and Correct Answers
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Course
BIO 2420
Institution
BIO 2420
What is the single most important way to reduce the spread of unwanted microbes? handwashing
Where and how should used media (culture tubes and plates) be disposed of? directly into bio hazard bin
Where and how should a slide prepared by you (ex: Gram stain) be disposed of? Wrapped in a paper tow...
BIOL 2420 Lab Practical 1 Test
Questions and Correct Answers
What is the single most important way to reduce the spread of unwanted microbes?
✅handwashing
Where and how should used media (culture tubes and plates) be disposed of?
✅directly into bio hazard bin
Where and how should a slide prepared by you (ex: Gram stain) be disposed of?
✅Wrapped in a paper towel and disposed into the biohazard bin
Where should gloves worn by you (after lab, not cleaning up a spill) be disposed of?
✅in the regular trash can
Where should a broken stock slide (ex: 3 forms slide that was observed during lab 3) be
disposed of? ✅in the broken glass container
What are the last two things that you always do before leaving the microbiology lab that
will ensure that you do not spread organisms used in the lab out of the lab and will also
ensure that the next class coming into the lab will not be contaminated? ✅1. Disinfect
lab bench
2. Wash hands
Select all required information needed to properly label your cultures when labeling
culture media. ✅1. Name of medium (TSA, TSB)
2. Date of inoculation
3. The inoculum (microbes name)
4. Your initials (F, M, L)
Liquid media ✅media that does not contain agar
Solid media ✅media that contains the solidifying agent, agar, in it
Incinerator ✅Equipment used to sterilize the inoculating loops via incineration
Inoculate ✅Introduce cells or organisms into a culture medium
Inoculum ✅Organism or material used for inoculation
Incubator ✅Equipment used to grow microorganisms in a controlled environment
, Why is it incorrect to label a culture tube cap or the lid of the petri dish? ✅1. Lids can
easily fall off or be switched.
2. Plate media is incubated upside down
Identify where you would place the culture in the following scenario:
You inoculated a broth with E. Coli in lab and want to grow the culture for 48 hours and
will check on the culture during the next lab period. ✅37 degrees C
Identify where you would place the culture in the following scenario:
You inoculated a broth with E. Coli in lab but did so at the end of the week. You won't be
back in lab until after the weekend. Therefore, the growth needs to be slowed down as
the incubation time will be longer than 72 hours. ✅25 degrees C
Identify where you would place the culture in the following scenario:
You have observed your results but want to store your culture for future observations.
✅4 degrees C refrigeration
Observe Figure 2-1. Microorganisms are everywhere in the environment. Select two
beneficial roles that microorganisms play in the environment. ✅1. Decomposers
2. Producers
Microorganisms are found everywhere on body surfaces and lining lumens. What four
beneficial roles do microorganisms play as microbiota? ✅1. Exclude pathogens from
colonizing
2. Aid in digestion
3. Synthesize Vitamins K & B12
4. Stimulate immune response
Why is it important as a beginning microbiologist to appreciate the ubiquity of
microorganisms when working in a microbiology lab and trying to maintain aseptic
technique? ✅.To be aware that microorganisms are everywhere and can very easily
contaminate your cultures
Select the two steps you need to follow to properly handle the microscope power cord
when you are finished. ✅1. Wrap the cord around the microscope base
2.Hold the plug and remove it from the outlet
Select the two steps you need to follow to properly put away prepared stock slides when
you are finished. ✅1. Wipe fingerprints and oil off with a paper towel
2. Return slide to designated tray
Select the two steps you need to follow to properly put away student stained slides
when you are finished. ✅1. Wrap slide in a paper towel
2. Throw slide away in biohazard container
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