LAB PRACTICAL 1 - BIOL 2420 QUESTIONS AND CORRECT ANSWERS
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Course
BIO 2420
Institution
BIO 2420
Define ubiquity of microorganisms. Basically that microorganisms can be found/grown anywhere in almost every type of environment
GloGerm activity purpose to train you to wash your hands more thoroughly/effectively; shows that hand washing with soap is not enough to remove all microbes
How do you ...
LAB PRACTICAL 1 - BIOL 2420
QUESTIONS AND CORRECT ANSWERS
Define ubiquity of microorganisms. ✅Basically that microorganisms can be
found/grown anywhere in almost every type of environment
GloGerm activity purpose ✅to train you to wash your hands more
thoroughly/effectively; shows that hand washing with soap is not enough to remove all
microbes
How do you focus a microscope? ✅4x (scanning) - coarse knob - red ring
10x (low) - fine knob - blue ring
40x (high) - fine knob - yellow ring
100x (immersion oil) - fine knob - black/white rings
When do you use immersion oil objective? ✅to see greater detail in
bacteria/microorganisms
How to calculate total magnification ✅ocular lens x objective lens
for us: 10 x (4;10;40;100)
Options: 40/100/400/1000
Microscope parts ✅Stage
Ocular lens
Objective lens
body
revolving nose piece
stage clip
iris diaphragm
condenser
illuminator
kholer illumination
Mechanical stage adjustment knobs
speciman holder
Fine/coarse focus knobs
reostat knob
What are the creatures in the pond water? ✅Protozoans
What are protozoans? ✅the single celled eukaryotes that live in pond water
, What is a bacterial colony? ✅a cluster of cells that originates from the multiplication of
a single cell and replicates and represents the growth of a single species of
microorganisms growing on a solid surface.
Why do we use aseptic technique? ✅For safe handling and to avoid contamination.
Streak for isolation ✅procedure used to isolate pure colonies, generally from a mixed
colony on an agar plate
creating a bacterial lawn ✅evenly spreading sample across entire agar plate, making
sure to turn the plate 1/4 turn 3-4 times to ensure that the sample has been evenly
dispersed, so as to grow evenly across entire plate
How to use a microscope ✅raise the substage condenser
use nosepiece ring to move to scanning objective
4x - focus using coarse focus knob
adjust diaphragm
turn to 10x objective - parfocal
focus using fine focus knob
turn to 40x objective - parfocal
focus using fine focus knob
turn nosepice half way
add oil
slide 100x (oil immersion) into place
use fine focus knob
NEVER go back to dry objectives after oil has been added
Cocci ✅spherical bacteria
Bacillus ✅Rod shaped bacteria
strepococci ✅spherical bacteria that form a chain
Staphylococcus ✅rod shaped bacteria in a chain
Spirilla ✅spiral shaped bacteria
Antibiotics ✅natural antimicrobial agents produced by microorganisms
antimicrobials ✅Synthetic agents used to treat bacterial infections
Kirby-Bauer test ✅standardized disk diffusion method of testing the effectiveness of
multiple antimicrobial chemicals on one agar plate
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