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Uric Acid measurement Questions and Answers
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Uric Acid measurement Questions and Answers · Principle: Reduction-Oxidation (Redox) Reaction · Oxidation of uric acid in a protein free filtrate (It is required to remove protein in this method) · Reduction of phosphotungstic acid · Based on the oxidation of uric acid in a protein-free f...

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  • September 30, 2024
  • 4
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers

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  • uric acid measurement questions and answers
  • principle reduction oxidation redox reaction
  • uses sodium carbonate instead of sodium cyanide

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  • ASID

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Uric Acid measurement Questions and
Answers
· Principle: Reduction-Oxidation (Redox) Reaction
· Oxidation of uric acid in a protein free filtrate (It is required to remove protein in this
method)
· Reduction of phosphotungstic acid

· Based on the oxidation of uric acid in a protein-free filtrate with subsequent reduction
of phosphotungstic acid in alkaline solution to tungsten blue

· Lacks specificity - answer Phosphotungstic Acid (Caraway Method) Principle

· Uses sodium carbonate instead of sodium cyanide**
o Provides alkalinity [For color development]
o Allantoin will be the reducing agent to reduce phosphotungstic acid to tungsten
o **The traditional method uses sodium cyanide; Toxic - answer Henry's Modification
of the Caraway Method

· Uric acid + phosphotungstic acid + O2 --NaCO3 /OH> tungsten blue + allantoin + CO2

NaCO3 /OH = Used to create an alkaline environment; Inhibits reducing substances
(ascorbic acid, glucose, cysteine); Used for color development - answer Equation for
caraway method

False increase = Turbidity, aspirin and metabolite, acetaminophen, caffeine,
theophylline - answer Interferences caraway

Blauch and Koch Method
First Step (Uricase)
· Principle: Enzymatic production of allantoic from uric acid
· In the presence of uricase/urate oxidase, uric acid is converted to allantoin and
hydrogen peroxide
· Uricase is an enzyme that hastens the oxidation of uric acid to allantoin
· Used almost exclusively; More specific than Caraway
· Measured at 293 nm
i. Uric acid has a UV absorbance peak at 293 nm
ii. Allantoin does not have a UV peak at that wavelength
a. The difference in absorbance before and after incubation with uricase is proportional
to the uric acid concentration - answer Enzymatic Method A. Spectrophotometric

· Proteins can cause high absorbance and decreases sensitivity

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