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CSMLS Instrumentation Questions And Answers With Verified Solutions Latest Updated 2024
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CSMLS Instrumentation Questions And Answers With Verified Solutions Latest Updated 2024
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CSMLS Instrumentation Questions AndAnswers With Verified Solutions Latest
Updated 2024
The ability of the microscope to separate small details is defined as:
a. magnification
b. definition
c. resolution
d. numerical aperture - ANSWER✔✔ c. resolution is the ability to distinguish
between adjacent details or to reveal fine detail. resolving power is measured as the
least distance between 2 objectives at which the objects can be discerned as 2
separate structures rather than a single blurred object
Sections 90-nm thick are commonly cut with a/an:
a. sliding microtome
b. ultramicrotome
c. rotary microtome
d. vibratome - ANSWER✔✔ the ultramicrotome is used to cut 90nm sections for
electron microscopy
When cutting sections from paraffin blocks, the most common cause of
unsatisfactory sections is:
a. poor fixation of the tissue
b. a flotation bath that is too hot
c. paraffin with a low melting point
d. a poor blade edge - ANSWER✔✔ d. poor blade edge is the most common cause
(can also be caused by poor fixation)
, The regular laboratory incubator maintains a temperature of about:
a. 4 C
b. 28 C
c. 37 C
d. 60 C - ANSWER✔✔ c. 37 C
When the magnification can be changed without the need to refocus, the
microscope objectives are said to be:
a. parfocal
b. binocular
c. achromatic
d. apochromatic - ANSWER✔✔ a. when objectives are parfocal, all objectives will
have the focal point in the same plane and magnification can be changes without
the need to refocus
If a ribbon splits when cutting paraffin sections, the trouble is most likely due to:
a. too much tilt in the knife
b. nicks in the knife
c. static electricity
d. a loose block - ANSWER✔✔ b. nicks in the edge of the blade are the most
common cause of split paraffin ribbons
Care must be taken when using automatic coverslippers to ensure that:
a. only no. 1 coverslips are applied
b. only full baskets of slides are loaded on the coverslipper
c. the mounting medium applied is correct for long-term storage
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