BIOCHEM ACS STUDY GUIDE WITH VERIFIED ANSWERS
1. B
2. C
3. B
4. B
Each of the following subcellular structures contains DNA
EXCEPT
A. the nucleus.
B. ribosomes.
C. chloroplasts.
D. mitochondria.
In plant and animal cells, the three-dimensional integrity
of the cell
is maintained, in pa...
1. B Each of the following subcellular structures contains DNA
EXCEPT
A. the nucleus.
B. ribosomes.
C. chloroplasts.
D. mitochondria.
2. C In plant and animal cells, the three-dimensional integrity
of the cell
is maintained, in part, by an internal network of filaments
called
A. the cell wall.
B. the Golgi apparatus.
C. the cytoskeleton.
D. the endoplasmic reticulum.
3. B Of the following, which are prokaryotes?
A. viruses
B. bacteria
C. yeast
D. plant cells
4. B G° for the conversion of glycerol-3-phosphate to glycerol
+ Pi is -2.2 kcal/mole. Thus, you can conclude that
A. this reaction will occur spontaneously.
B. this reaction may or may not occur spontaneously,
depending on the
actual concentrations of reactants and products.
C. this reaction will only occur spontaneously if it is cou-
pled to
another reaction that has a more favorable G°.
D. this reaction is so strongly favorable that it could be
coupled to
drive an otherwise unfavorable cell reaction.
5. A
, BIOCHEM ACS STUDY GUIDE WITH VERIFIED ANSWERS
In most biochemical reactions, PV is
A. negligible
B. positive
C. negative
D. dependent on E
6. A According to the Second Law of Thermodymnamics,
A. the total entropy change in any reaction that occurs
spontaneously must be greater than 0.
B. the total entropy change in any reaction that occurs
spontaneously must be less than 0.
C. the total entropy of the universe remains constant.
D. the total entropy of the universe is always decreasing.
7. C Which of the following is true?
A. Liquid and solid water have the same number of
hydrogen bonds per molecule of water
B. Liquid water has more hydrogen bonds per molecule
than does solid water
C. Solid water has more hydrogen bonds per molecule
than does liquid water
D. Hydrogen bonding is a major factor in the structure of
gaseous water
E. None is true
8. D What is the pH of an aqueous solution that has a [OH-] of
3.45 x 10-5 M?
A. 3.47
B. 4.47
C. 5.53
D. 9.54
E. none of the above
9. D A peptide was found to have a molecular mass of about
650 and upon hydrolysis produced Ala, Cys, Lys, Phe,
and Val in a 1:1:1:1:1 ratio. The peptide upon treatment
with Sanger's reagent produced DNP-Cys and expo-
sure to carboxypeptidase produced valine. Chymotrypsin
treatment of the peptide produced a dipeptide that con-
, BIOCHEM ACS STUDY GUIDE WITH VERIFIED ANSWERS
tained sulfur and has a UV absorbance, and a tripeptide.
Exposure of the peptide to trypsin produced a dipeptide
and a tripeptide. Deduce the sequence of the peptide.
A. Val-Ala-Lys-Phe-Cys
B. Cys-Lys-Phe-Ala-Val
C. Cys-Ala-Lys-Phe-Val
D. Cys-Phe-Lys-Ala-Val
E. Val-Phe-Lys-Ala-Cys
10. B Which amino acid has an R-group pKa closest to physio-
logical pH?
A. lysine
B. histidine
C. glutamate
D. arginine
E. aspartate
11. C Which is true regarding the orientation of the R-groups in
beta structures?
A. In the parallel beta sheet structures the amino acid
R-groups are all on the same side of the sheet.
B. In the antiparallel beta sheet structure the R-groups on
alternate strands are on the same side of the sheet.
C. In parallel and antiparallel sheets consecutive
R-groups on each peptide strand alternate sides of the
sheet.
D. Because of the free rotation around the alpha carbon,
the R-groups can seek the least crowded region and are
not restricted to specific side of the sheet.
E. a and b
12. C A reagent commonly employed to cleave disulfide bonds
in proteins is
A. 2,4-dinitrofluorobenzene
B. urea
C. 2-mercaptoethanol
D. phenylisothiocyanate
E. cyanogen bromide
13. D
, BIOCHEM ACS STUDY GUIDE WITH VERIFIED ANSWERS
With respect to a relaxed muscle, which of the following
is true?
A. the number of actin-myosin cross-bridges is maxi-
mized and the muscle is shortened
B. the number of actin-myosin cross bridges is maximized
and the muscle is stretched
C. the number of actin-myosin cross bridges is minimized
and the muscle is shortened
D. the number of actin-myosin cross bridges is minimized
and the muscle is stretched
E. none of the above
14. D Which of the following does not describe hemoglobin?
A. Hemoglobin is an oxygen transport protein.
B. Hemoglobin is an allosteric protein.
C. Hemoglobin has quaternary structure.
D. Copper ions are important in hemoglobin's ability to
bind oxygen.
E. Hemoglobin is composed, in part, of an iron containing
heme group.
15. B Which of the following would allow you to determine the
isoelectric
point of a protein?
A. protein quaternary structure
B. protein solubility as a function of pH
C. protein size
D. protein shape
E. protein tertiary structure
16. B A mixture of urease (pI = 5.1, mol. wt. 482,700), catalase
(pI = 5.6 mol. wt. 247,500), lactoglobin (pI 5.2, mol. wt.
37,100) and hemoglobin (pI 6.9, mol. wt. 64,500) were
applied in a pH 6.5 buffer to a DEAE-cellulose chromatog-
raphy column and eluted with the same buffer.
What was their order of elution?
A. urease, lactoglobin, catalase, hemoglobin
B. hemoglobin, catalase, lactoglobin, urease
C. urease, catalase, hemoglobin, lactoglobin
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