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Microbiology Csmls Prep Questions And Answers With Verified Solutions Already Passed!!!(GRADED A+) $15.49   Add to cart
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Microbiology Csmls Prep Questions And Answers With Verified Solutions Already Passed!!!(GRADED A+)
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Microbiology Csmls Prep Questions And Answers With Verified Solutions Already Passed!!!(GRADED A+)

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  • September 28, 2024
  • 81
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers

Subjects

  • microbiology csmls prep questions and answers with
  • if there is no zone of inhibition what do you rec
  • what causes sharp irregular zones around the sensi
  • susceptible vs intermediate vs resistant

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  • CSMLS
  • CSMLS

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Microbiology Csmls Prep Questions And
Answers With Verified Solutions Already
Passed!!!(GRADED A+)
if there is no zone of inhibition, what do you record? - ANSWER✔✔ 6mm
because that accounts for the disc


what causes sharp irregular zones around the sensitivity discs? - ANSWER✔✔
beta-lactam antibiotics


- indicates that beta-lactamase production by the bacteria and should be interpreted
as resistant


susceptible vs intermediate vs resistant - ANSWER✔✔ - susceptible = susceptible
to action of the antimicrobials and is stopped or killed by it


- intermediate = used for certain drug combinations that are difficult to test or
produce results of certainty


- resistant = resistant to the action of antimicrobial and keeps growing in its
presence


QC for susceptibility tests - ANSWER✔✔ - each time new discs or panels are
received
- each time tests are performed (once a week)
- organisms are staph aureus, E.coli, pseudo, enterococcus

,how should antimicrobial discs be stored? - ANSWER✔✔ - long term = frozen at -
20C
- working supply in 4C


MRSA conditions - ANSWER✔✔ - addition of NaCl
- 30-35C (lower temp is better)
- displays trailing on disc diffusion methods
- healthcare workers are screened for it
- use oxacillin screen test


Haemophilus test medium (HTM) is supplemented with - ANSWER✔✔ - bovine
hematin
- yeast extract
- NAD
- thymidine phosphorylase


E-test is beneficial for using it on - ANSWER✔✔ - anaerobes
- fastidious bacteria


E-test results interpretation: low MIC and high MIC - ANSWER✔✔ low MIC -
sensitive


high MIC - resistant


Staphylococcus aureus characteristics - ANSWER✔✔ - double zone of beta
hemolysis

,- creamy yellow color from lipochrome @48H
- ferments mannitol (yellow)
- catalase positive
- nitrate positive
- slide or TUBE coagulase positive


MSA media (mannitol salt agar) - ANSWER✔✔ - 7.5% NaCl
- differential = mannitol and phenol red
- if it ferments mannitol it will be yellow, if it does not then it will be red
- staph aureus ferments (yellow)


MRSA oxacillin screen test - ANSWER✔✔ - 6ug oxacillin
- 4% NaCl
- any growth on plate = resistant


slide coagulase vs tube coagulase - ANSWER✔✔ - slide = bound coagulase


- tube = free coagulase


false negative coagulase tests are commonly from - ANSWER✔✔ weak bacterial
suspensions


gold standard of staph aureus is - ANSWER✔✔ positive tube coagulase

, what is found on the cell wall of S. aureus but not other staph species? -
ANSWER✔✔ Protein A and clumping factor = used in latex reagent methods


thermostable nuclease test is used for - ANSWER✔✔ ID of staph aureus
- food microbiology investigations of food poisoning


staphylococcus saprophyticus ID - ANSWER✔✔ - bright white opaque colonies
- catalase positive
- nitrate positive
- novobiocin resistant


- common UTI


what virulence factors does staphylococcus aureus have? - ANSWER✔✔ -
leucocidin
- hyaluronidase
- staphylokinase (clots)
- hemolysins


_______ is the most common cause of skin, soft tissue and post traumatic
infections - ANSWER✔✔ S. aureus


three diseases caused by S. aureus exotoxins - ANSWER✔✔ 1) food poisoning -
heat stable enterotoxin (fast)
2) toxic shock syndrome - tampons
3) scalded skin syndrome - rash & desquamation of skin

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