Exam (elaborations)
Csmls Histology Questions And Answers With Verified Solutions Already Passed!!!
- Course
- Institution
Csmls Histology Questions And Answers With Verified Solutions Already Passed!!!
[Show more]
Content preview
Csmls Histology Questions AndAnswers With Verified Solutions Already
Passed!!!
3 types of Carbohydrates demonstrated in tissues - ANSWER✔✔ Glycoproteins,
polysaccharides, proteoglycans
What stain is used for glycogen? - ANSWER✔✔ PAS - because glycogen has
vicin diols
Where is glycogen found in the body? - ANSWER✔✔ Liver, skeletel and cardiac
muscle, hair follicles, vaginal and ectocervical epithelium, mesothelium cells,
neutrophils and megakaryocytes.
What accounts for the staining reaction of Glycoproteins? - ANSWER✔✔ sialic
acid goups and vicin diols (PAS), also contains carboxyl acid group (Alcian Blue).
Where are glycoproteins in the body? - ANSWER✔✔ Most are associated with
epithelial tissues. They are classified as either neutral or acidic depending on
whether they contain a Sulphate or Carboxylate residues.
What do neutral glycoproteins lack? Where do you see them? - ANSWER✔✔
They lack bother Carboxylate (COOH) and Sulphate (SO3H). They are found in
goblet cells in the stomach as well as paneth cell granules in the small bowel.
Where are acidic glycoproteins that contain only COOH found in the body? -
ANSWER✔✔ They can be found submaxillary gland, small intestine, fetal
extracellular matrix, upper part of colonic crypts, sublingual gland.
, Where are acidic glycoproteins that contain both COOH and SO3H found in the
body? - ANSWER✔✔ Mainly found in colonic goblet cells.
What are Proteoglycans (Glycosaminoglycans)? - ANSWER✔✔ They are large,
unbranched carbohydrates with small poly peptide chains.
Where in the body can you find Proteoglycans? - ANSWER✔✔ Connective tissues
such as cartilage, bone, tendons, ect. Referred to as acid or neutral mucins.
What compound reacts with Cationic dyes in a Carbohydrate? - ANSWER✔✔ The
acid containing sugar (COOH or SO3H)
What are the routine stains for Carbohydrates ? - ANSWER✔✔ PAS, Alcian Blue
Muricarmine, Colloidal iron, Metachromasia, Lectins
2 main steps in PAS method? - ANSWER✔✔ Oxidize carbohs with periodic acid
to create aldehydes.
Demonstrating the resulting aldehydes with Schiffs reagent.
Potential false PAS positives ? - ANSWER✔✔ When glutaraldehyde is used as a
fixative, and only some lipids found in frozen sections.
What does Schiffs reagent do in PAS? - ANSWER✔✔ It is attracted to an reacts
with aldehydes.
Some Proteoglycans contain vicin diols but do not stain with PAS, why ? -
ANSWER✔✔ Because the periodate ion is repelled by the COO and SO3,
therefore oxidation does not occur and the aldehydes are not exposed.
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller classhub. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $12.99. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
75860 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now