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MIC 205 Exam 1 – 102 Questions And Answers (ASU Lisenbee MIC 205)
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MIC 205 Exam 1 – 102 Questions AndAnswers (ASU Lisenbee MIC 205)
Antoni van Leeuwenhoek - - "Father of Protozoology" and
"Father of Bacteriology"
-Examined water and visualized tiny animals, fungi, algae, and
single celled protozoa; "animalcules"
- Carolus Linnaeus - - Grouped the 5 categories of microorganisms
- 5 categories of microorganisms - - -Small animals
- Fungi
- Protozoa
- Algae
- Prokaryotes
- Fungi - - eukaryotic, cell walls, get food from other organisms (mold,
yeast)
- Protozoa - - single celled eukaryotes, live in animal hosts
- Locomotion in Protozoa - - psudopodia
cilia
flagella
- Algae - - (unicellular or multicellular) Photosynthetic, categorized by
pigmentation, storage products, and cell wall
- Prokaryotes - - unicellular, NO nuclei
reproduce asexually
2 kinds- bacteria & archaea
- Viruses - - NOT cells, made of protein and DNA/RNA
require a host for replication
- Principles of microscopy - - wavelength, refraction/magnification,
resolution/contrast
- Refraction - - bending of light (radiation)
- Magnification - - perceived enlargement of an image
- Resolution - - capability of distinguishing between 2 adjacent objects
, - Contrast - - difference in intensity between 2 objects or an object and a
background
- Electron microscope limits - - can see proteins, ribosomes, viruses
- Light microscope limits - - hard to see viruses but can see cells
- Dark field microscope - - light against dark background
- Phase contrast microscopes - - 3D against gray background
- Fluorescence microscope - - colored against black background
- Confocal microscopes - - removes out of focus light, can be stacked to
make a 3D image
- Electron Microscopes - - view bacteria, viruses, internal cell structure,
molecules and large atoms
- Transmission electron microscope - - Cross sections of cells/viruses to see
internally
- Scanning electron microscopes - - surface views of organisms
- Scanning probe microscopes - - see single molecules and large atomic
clusters
- Staining - - increase contrast and resolution with dyes
-simple, differential, special
- Gram stain - - differential stain
1. crystal violet (purple dye)
2. iodine (mordent)
3. alcohol (decolorize)
4. safranin (red dye)
- Gram stain turns gram positive what color? - - Purple
- Gram stain turns gram negative what color? - - Pink
- Domains of basic types of cells - - eukarya
bacteria
archaea
- Prokaryotes - - no nucleus, bacteria and archaea
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