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med lab midterm 1 review exam //All Chapters With 100% Correct Answers / A + Grade.

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med lab midterm 1 review exam //All Chapters With 100% Correct Answers / A + Grade. Specifications (printed on the stem of the pipette): - CORRECT ANSWERS-Maximum volume that can be transferred Size of divisions on the pipette What is the smallest volume that can be delivered accurately in thi...

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  • September 24, 2024
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  • 2024/2025
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med lab midterm 1 review exam //All Chapters
With 100% Correct Answers / A + Grade.

Specifications (printed on the stem of the pipette): - CORRECT ANSWERS-Maximum
volume that can be transferred

Size of divisions on the pipette

What is the smallest volume that can be delivered accurately in this pipette? -
CORRECT ANSWERS-0.1 mL

Glass Pipettes - CORRECT ANSWERS-Volumetric (transfer) vs. Graduated
(measuring)

volumetric pipet - CORRECT ANSWERS-Calibrated to deliver a fixed volume of liquid
by drainage

Calibration Mark etched on stem, "bulb" in middle, gradual taper at tip

To be used when accuracy is required to deliver an exact amount

For ex: a 5 mL Volumetric pipette has a tolerance of 0.01 mL

Ostwald Pipette - CORRECT ANSWERS-similar to volumetric but with bulb closer to the
tip. Used for viscous fluids (ex. serum)

Graduated - CORRECT ANSWERS-Calibrated to deliver a volume of liquid between 2
calibration marks

an use to transfer various volumes

To be used when speed is required

Less accurate than volumetric pipettes

Ex: a 5 mL Graduated pipette has a tolerance of 0.02 mL

Two types of graduated pipettes - CORRECT ANSWERS-Mohr pipet - CORRECT
ANSWERS-Precipitate (pellet): the packed particles (solid) at the bottom of a
centrifuged tube

Supernatant: the liquid portion at the top of a centrifuged tube

,Decantation: separation of the supernatant from the pellet

- CORRECT ANSWERS-Rotor and carriers attached to the vertical shaft of a motor
and enclosed in a metal covering

Centrifuge: - CORRECT ANSWERS-apparatus used to separate substances of different
density or particle size, when suspended in a fluid, by spinning them about an axis in a
suitable container

Rotor and carriers attached to the vertical shaft of a motor and enclosed in a metal
covering

Rotor (centrifuge head): - CORRECT ANSWERS-Primary component of a centrifuge
which holds the material to be centrifuged and which is rotated by the drive system.

Centrifuges classified according to the following: - CORRECT ANSWERS-Benchtop vs.
floor

Refrigerated or not refrigerated

Rotor head type (fixed, hematocrit, swinging-bucket, angled)

Maximum speed attainable

Separating serum or plasma from blood cells

Normal separation of serum from clot: - CORRECT ANSWERS-Min of 1000 g for 10
min

Relative Centrifugal Force (RCF): - CORRECT ANSWERS-The force required to
separate 2 phases in a centrifuge.

Based upon 3 variables: Mass, speed, and radius

Expressed as #times greater than gravity

- CORRECT ANSWERS-RCF = 1.118 x 10-5 x r x rpm

Where:r = radius (cm) from center of rotation to bottom of tube in the rotor cavity

rpm = speed of rotation of the rotor in revolutions per minute

What is the RADIUS in a centrifuge? - CORRECT ANSWERS-

Horizontal-head - CORRECT ANSWERS-(swinging-bucket):

,bucket moves to allow tubes to spin in a horizontal position and rest in a vertical position

Particles travel constantly along the tube

Sediment is distributed evenly (flat surface)
WHMIS - CORRECT ANSWERS-- is a national hazard wide communication system

- Created to inform workers of the hazardous materials they work with

Why was WHMIS created? - CORRECT ANSWERS-- inform about the safety and
health hazards associated with the hazardous materials they might use at work

- Reduce injuries and illnesses associated with handling hazardous materials in the
workplace

GHS - CORRECT ANSWERS-- Global Harmonized System

-Move to standardize communication about hazardous products internationally

GHS consists of... - CORRECT ANSWERS-- Health hazards
- Physical hazards
- Environmental hazards (NOT ADOPTED BY CANADA)

WHMIS 2015 - CORRECT ANSWERS-- Canada's response to Globally Harmonized
System (GHS)

Consists of

- Labels (provides info about product and hazards)
- SDS (detailed info about hazards)
- Education (Training on how to safely handle hazardous products)

Suppliers - CORRECT ANSWERS-Classifying products

Establishing health and safety information

Labelling products

Preparing and providing SDS's

Employer - CORRECT ANSWERS-Ensure proper labels

Provide access to SDS's and update

Educate workers, and evaluate competency

, Train workers on how to handle, use, store, wear PPE for hazardous products, assess
competency

Provide control measures, adhere to Routine practices

Employees - CORRECT ANSWERS-Participating in education and training

Following prescribed safe work procedure

Identifying and controlling hazards

Hazardous Product - CORRECT ANSWERS-- Hazardous products are materials,
products, or substances that meet any of the criteria for one or more of the 32 GHS
Hazard Classes as defined in the Federal Hazardous Products Regulation

- Grouped into physical and health hazards

- WHMIS does not provide a comprehensive list of hazardous products, only a list of
hazard criteria.

Exclusions from WHMIS - CORRECT ANSWERS-- WHMIS may not apply if another act
or regulation is in place

- The following are exempt from the supplier responsibilities of WHMIS (no label or SDS
required):

•Consumer restricted products: products sold in stores that already have labels in
accordance with the Canada Consumer Product Safety Act

•Explosives: covered by the Explosives Act

•Cosmetics, drug, food, or devices: covered by the Food and Drugs Act

•Pesticides, herbicides, and insecticides: covered by the Pest Control Products Act

- Employer still responsible for for training employees on the safe use, handling, and
storage of these materials

more exceptions - CORRECT ANSWERS-Radioactive materials: covered by the
Nuclear Safety and Control Act

Wood and wood products•Manufactured articles that will not release chemicals

•Tobacco or tobacco products•In-house generated hazardous waste

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