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BIOE 206 EXAM 1 STUDY GUIDE QUESTIONS AND ANSWERS
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BIOE 206 EXAM 1 STUDY GUIDE QUESTIONS AND ANSWERS
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Name: Score:111 Multiple choice questions
Term 1 of 111
What do prokaryotes have in transcription that eukaryotes don't?
The transcription of RNA into DNA using reverse transcriptase.
Enhancers, activators, and accessory proteins.
Glycine, Alanine, Valine, Leucine, Methionine, and Isoleucine.
The use of methods to study and manipulate information about the central dogma.
Term 2 of 111
What is the role of polymerase I, II, IV, and V.
They recognize different sites.
two or more polypeptide chains joined
The amino acid sequence.
Error checking and repair.
Term 3 of 111
What is the role of the threshold cycle value?
two or more polypeptide chains joined
The transcription of RNA into DNA using reverse transcriptase.
To show the number of cycles required for the fluorescent signal to exceed the
background.
They are flexible, have a high degree of rotation, hydrophobic, and usually buried in the
interior of the protein (away from water).
,Term 4 of 111
What are the properties of the amino acids in the positively charged R group?
G-C is more stable because it has more hydrogen bonds, which creates a stronger
resistance to denaturation.
They are positively charged, typically solvent exposed, and are able to hydrogen bond
and from electrostatic interactions.
They are constrained, have large hydrophobic surface areas, have pi-pi interactions, and
absorb ultraviolet light.
The backbones are further apart in the major grooves. Proteins can interact with bases
easier in major grooves.
Term 5 of 111
3-letter and one letter code for Cysteine
Cys, C
Asn, N
Ala, A
Gln, Q
Term 6 of 111
What is PCR used for?
To show the number of cycles required for the fluorescent signal to exceed the
background.
they use reverse transcriptase to make DNA from RNA
To make copies of (amplify) small sections of DNA or genes.
A basic unit of chromosome, they are made up of nucleosomes.
,Term 7 of 111
-is a substance within - consisting of DNA and protein. The major proteins in - are -, which help
package the DNA is a compact form that fits in the - -
Ionic, van der Waals attractions, and hydrogen
Continuous because replication proceeds in the 5' to 3' direction on the leading strand.
There is a tube that you just hope as a lot of your fabric.
Chromatin, chromosome, chromatin, histones, cell nucleus.
Term 8 of 111
What is extending?
When the temperature is raised and the new strand of DNA is made by the Taq
polymerase enzyme.
They are flexible, have a high degree of rotation, hydrophobic, and usually buried in the
interior of the protein (away from water).
They are constrained, have large hydrophobic surface areas, have pi-pi interactions, and
absorb ultraviolet light.
Prokaryotes have supercoiling and the promoters wouldn't work naturally in a eukaryotic
system.
, Term 9 of 111
How does eukaryotic transcription work?
They are constrained, have large hydrophobic surface areas, have pi-pi interactions, and
absorb ultraviolet light.
Beta sheets that formed from neighboring segments of the polypeptide backbone that
are parallel chains or form a polypeptide backbone that folds back and forth upon itself
with each chain antiparallel to the next. Alpha helix is generated when a single
polypeptide chain twists around on itself to form a rigid cylinder. A hydrogen bond
forms between every fourth peptide bond linking the C=O of one peptide bond to the
N-H of another.
There are 3 RNA polymerases. RNA P1 transcribes genes 5.8s, 18s, and 28s rRNA genes.
RNA P2 transcribes all protein coding genes plus most snRNA genes. RNA P3 transcribes
tRNA genes, 5s rRNA genes, and other small RNAs.
They are polar, relatively flexible, and tend to be surface exposed for hydrogen bonding
with water and other functional groups.
Term 10 of 111
What does DNA polymerase III do?
They can pass through membranes or be anchored by membranes.
Adds new base pairs complementary to the strand during replication
A scaffold or structure. They can recognize different things to allow things in.
The larger the S value of rRNA, the larger the rRNA.
Term 11 of 111
What are the primary polymerases involved in DNA replication in eukaryotic cells?
The amino acid sequence.
They are polar, relatively flexible, and tend to be surface exposed for hydrogen bonding
with water and other functional groups.
alpha, delta, and epsilon
1. Seuquence specific DNA binding
2. Sequence non-specific DNA binding.
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