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Lectures 1-3 Study Questions EBME 306
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Lectures 1-3 Study Questions EBME 306
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Name: Score:36 Multiple choice questions
Term 1 of 36
What are gross morphological findings?
They are descriptive words like its "liver-like, hard soft, fibrotic or necrotic.
They are like saying it is fibrotic, calcific, neoplastic, apoptocic.
They are morphologic You can have gross descriptive findings, or you can have
microscopic descriptive findings.
Advantage:
Semi quantifiable depending on band intensity
Disadvantage:
Cells lysed prior to examination
Application:
Commonly used in cell culture research
,Term 2 of 36
What are some of the advantages and disadvantages and applications of western blotting
Advantage:
Semi quantifiable depending on band intensity
Disadvantage:
Cells lysed prior to examination
Application:
Commonly used in cell culture research
Advantages:
Specific localization of antigens in situ, staining lasts for years. Relatively inexpensive
Disadvantages:
Not suitible for light impenetrable materials, qualitative and subjective without computer
analysis
Applications:
Commonly used for the examination pathological samples e.g. disease diagnosis
Advantage
Visualization of cells on light impenetrable materials
Disadvantage:
Fluorescence fades over time, requires a relatively expensive fluorescent
Applications:
Widely used in cellular in vivo and in vitro
Advantage:
High magnification allowing sub-cellular location of antigens. Can be performed on
light impenetrable materials
Disadvantage:
Expensive and time consuming requires electron microscopy.
Application:
Allows the intra-cellular location of antigens.
,Term 3 of 36
What is a hemolysis test?
INVITRO TEST: Extract of material is prepared and evaluated for cytoxicity, usually done
in saline, PBS or other culture mediums.
Then add that to cultured cells and see how the cell responds over time. Rated from 0-4
with 4 being most severe
Exposure of material to animal blood (rabbit) for a period of time. about an hr, then
After centrifugation read supernatant for hemolysis (545nm) less than 5% hemolysis
indicates compatibility.
IN VITRO TEST
Tissue culture cells without an overlay of agar or agarose gel. Then add test materials in
direct contact with thr cells. Depends on diffusion in culture medium. Measure with the
stain over time
They are morphologic You can have gross descriptive findings, or you can have
microscopic descriptive findings.
Term 4 of 36
What are problems with the direct contact test?
IN VITRO TEST: Movement may cause injury, therefore sensitivity issue or
misinterpretation of toxic effect. Use with other tests
They are descriptive words like its "liver-like, hard soft, fibrotic or necrotic.
IN VITRO TEST
Tissue culture cells without an overlay of agar or agarose gel. Then add test materials in
direct contact with thr cells. Depends on diffusion in culture medium. Measure with the
stain over time
INVITRO TEST: Extract of material is prepared and evaluated for cytoxicity, usually done
in saline, PBS or other culture mediums.
Then add that to cultured cells and see how the cell responds over time. Rated from 0-4
with 4 being most severe
, Term 5 of 36
Define pathologic findings
The body responds to a biomaterial implant by having either an immune response,
inflammation, cell injury, hemostasis, thrombosis, wound healing
They are like saying it is fibrotic, calcific, neoplastic, apoptocic.
They are morphologic You can have gross descriptive findings, or you can have
microscopic descriptive findings.
Whether or not blood would clump on surface
Protein adsorption profile over time
Platelet adhesion profile over time
Term 6 of 36
What are microscopic findings
Exposure of material to animal blood (rabbit) for a period of time. about an hr, then
After centrifugation read supernatant for hemolysis (545nm) less than 5% hemolysis
indicates compatibility.
They are like saying it is fibrotic, calcific, neoplastic, apoptocic.
Whether or not blood would clump on surface
Protein adsorption profile over time
Platelet adhesion profile over time
They are descriptive words like its "liver-like, hard soft, fibrotic or necrotic.
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