Term 1 of 113
is the skin a favorable environment? Why?
1. Isolate DNA to be cloned
2. Use restriction enzymes to "chop up" the DNA into fragments
3. Insert the fragments into a cloning vector, creating recombinant DNA
4. Insert the recombinant DNA into a new host, such as E.coli
5. Culture host cell, growing many identical copies
The skin is unfavorable for the colonization of most microorganisms
Because of the slightly acidic pH, high concentration of salt, and lack of moisture
Assistance in digesting food
Regulation of immune system
Prevention of colonization by pathogens
Production of essential vitamins
They provide the most robust analysis of microbial evolution
Term 2 of 113
what are some examples of physiological and biochemical features used to classify and
identify organisms?
The strain that is usually one of the first strains discovered and is often the most fully
characterized and described, folds the species name
Carbon and nitrogen sources, cell wall constituents, nutritional type, motility, oxygen
relationships, photosynthetic pigments, fermentation products and optimum pH
A process using computers that determine where genes start and end to allow scientists
to locate genes on the genome
,Term 3 of 113
bioinformatics
study of the lipidome, entire set of lipid profile
Study of the transcriptome
The study of the genome using computers
study of metabolome, entire set of small molecule metabolites
Term 4 of 113
True or False: The normal microbiota is the same for all humans.
True
False
Term 5 of 113
why is the NGS method performed over Sanger method?
It is the most commonly used thermostable enzyme, and it comes from hydrothermal
vents the thermophilic bacterium Thermus aquaticus
it is cheaper snd faster
Organs involved in the production of urine and organs for reproduction
Low pH levels and secretion of bile and pancreatic juices
Term 6 of 113
commensalism
A loss of either photosynthetic pigments from the dinoflagellates or complete expulsion
of the dinoflagellates form the coral
determination about to which taxon an organisms belongs
one individual gains from the association, but the other is neither harmed nor benefited
the relationship in which some reciprocal benefit accrues to both partners; they depend
on each other for growth and survival
,Term 7 of 113
amensalism
one individual gains from the association, but the other is neither harmed nor benefited
one organism has negative impact on another organism
Any stable association of one organism with another
both organisms benefit but do not have to interact for survival
Term 8 of 113
What is the genitourinary tract?
Provides means for transferring a gene of interest to a host organism during the cloning
process
They recognize DNA and cleave specific four to eight base pair sequences
Organs involved in the production of urine and organs for reproduction
One that only causes disease under certain conditions
Term 9 of 113
metabolomics
same species with different antigenic properties (antibodies)
study of metabolome, entire set of small molecule metabolites
A loss of either photosynthetic pigments from the dinoflagellates or complete expulsion
of the dinoflagellates form the coral
two organisms attempt to use the same resource
, Term 10 of 113
nomenclature
determination about to which taxon an organisms belongs
The study of the genome using computers
science of biological classification
assignment of names to taxa
Term 11 of 113
glycomics
same species with biochemical and physiological differences
study of the glycome, entire set of sugars
the entire collection of mRNA transcripts
study of RNA found in environmental samples
Term 12 of 113
metatranscriptomics
The study of the genome using computers
the entire collection of mRNA transcripts
study of RNA found in environmental samples
study of metagenomes, genetic material recovered from environmental samples
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