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MIP 302 - Exam 1 2024 with Answers to Questions
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MIP 302 - Exam 1 2024 withAnswers to Questions
What are the different shapes of colonies? - ✔✔Circular, Irregular, Rhizoid
(spider like), Punctiform (dots)
What is an isolation streak? - ✔✔Diluting a bacterial sample across an agar plate
in order to isolate a bacteria
How do you perform an isolation streak? - ✔✔Inoculate the upper third of the agar
plate with the swab as your primary streak; use the inoculating loop to pull two or
three from the primary streak then finish off the streak in the second third of the plate;
use the inoculating loop to pull two or three times from the secondary streak then
finish off the streak in the third third of the plate
What is a fish tail streak? - ✔✔Moving in a waving pattern across the agar plate
Why is our bacteria incubated at 37 C? - ✔✔That is about the same as human
body temperature
Why are plates inverted before incubating? - ✔✔To avoid condensation from
dripping on our samples and smearing the growth which would ruin the isolation
Where should you always label your plates? Why? - ✔✔Label them on the bottom
so that if the agar and lid separated, you could still tell what the sample was
,What are the 5 kingdoms? - ✔✔Animalia, Plantae, Fungi, Protista, Monera
What kingdom(s) contain prokaryotes? - ✔✔Monera
What kingdom(s) contain eukaryotes? - ✔✔Animalia, Plantae, Fungi, Protista
What kingdom(s) contain microorganisms? - ✔✔Protista, Fungi, Monera
What kingdom do bacteria fall into? - ✔✔Monera
What kingdom do fungi fall into? - ✔✔Fungi
What kingdom do protists fall into? - ✔✔Protista
What are yeasts? - ✔✔Single celled fungi
What are molds? - ✔✔An aggregate of many fungi cells
What is a virus? - ✔✔Acellular; composed of protein and nucleic acid (either RNA
or DNA, but not both); obligate intracellular parasites
What is a prion? - ✔✔Composed of only protein; cause normal cellular protein
to refold and become prion protein
What are the three domains? - ✔✔Archaebacteria, Eubacteria, Eukarya
, Explain how to properly write a scientific name of a bacteria. - ✔✔Genus name
comes first and should be capitalized; species name comes second and should be lower
case; both names are italicized or underlined; the first time a name is used it should be
written out in full but after that, the genus name can be shortened to one letter
What is aseptic technique? - ✔✔Techniques used to prevent microbial contamination
What is the purpose of aseptic technique? - ✔✔To prevent microbial
contamination in our samples
What are the general basic rules of aseptic technique? - ✔✔Clean your work bench
of clutter; put on your lab coat; tie back long hair; wash your hands; disinfect your
bench with Wescodyne; work with open tubes near the flame to prevent entry of
airborne microbes; slant open tubes and plates away from your mouth and nose; never
lay caps, lids or culture tubes on the bench top; do not talk while tubes or plates are
open; never put anything in your mouth; work quickly; keep tubes and plates closed
when not in use; remove your lab coat before you leave; wash your hands
What are microscopic ocean-dwelling algae called? - ✔✔Phytoplankton
What is special about the cell walls of algae? - ✔✔They are rigid and made up of
silica or calcium carbonate which cause them to have symmetrical shapes
What is responsible for diatomaceous earth? - ✔✔Diatoms and their silica cell walls
What is a protozoan? - ✔✔Usually motile, eukaryotic, unicellular protist
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