100% satisfaction guarantee Immediately available after payment Both online and in PDF No strings attached
logo-home
MB (ASCP) EXAM ATCUAL EXAM 300 QUESTIONS AND CORRECT DETAILED ANSWERS (100% VERIFIED ANSWERS) $12.49   Add to cart

Exam (elaborations)

MB (ASCP) EXAM ATCUAL EXAM 300 QUESTIONS AND CORRECT DETAILED ANSWERS (100% VERIFIED ANSWERS)

 1 view  0 purchase
  • Course
  • 2024/2025
  • Institution
  • 2024/2025

MB (ASCP) EXAM ATCUAL EXAM 300 QUESTIONS AND CORRECT DETAILED ANSWERS (100% VERIFIED ANSWERS) Predominant form of DNA - Answer️️ -Right-handed B DNA and histone interaction is due to what type of bond? - Answer️️ -Ionic What stains to the minor groove of DNA duplex? - Answer️️ -S...

[Show more]

Preview 4 out of 32  pages

  • September 18, 2024
  • 32
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • 2024/2025
  • 2024/2025
avatar-seller
EmillyCharlotte
TITLE: EMILLYCHARLOTTE 2024/2025 ACADEMIC PERIOD
OWNER: EMILLYCHARLOTTE
COPYRIGHT STATEMENT: ©2024 EMILLYCHARLOTTE. ALL RIGHTS RESERVED
FIRST PUBLISHED: SEPTEMBER 2024

MB (ASCP) EXAM 2023-2024 ATCUAL
EXAM 300 QUESTIONS AND CORRECT
DETAILED ANSWERS (100% VERIFIED
ANSWERS)


Predominant form of DNA - Answer✔️✔️-Right-handed B


DNA and histone interaction is due to what type of bond? - Answer✔️✔️-Ionic

What stains to the minor groove of DNA duplex? - Answer✔️✔️-SYBR Green

What is the most abundant type of RNA? - Answer✔️✔️-Ribosomal RNA (rRNA)

What does RNA act as in a retrovirus? - Answer✔️✔️-Genome and mRNA

What increases half-life of mRNA? - Answer✔️✔️-5' Methyl Cap

What kind of DNA is most likely to be transcriptionally active? - Answer✔️✔️-Euchromatin

What is the most common type of eukaryotic DNA? - Answer✔️✔️-Satellite DNA

What specimen has the most DNA according to molecular weight? - Answer✔️✔️-White

Blood Cells

What amino acids compose histones? - Answer✔️✔️-Lysine and Arginine

Amino acid that all proteins start with: - Answer✔️✔️-Methionine

,TITLE: EMILLYCHARLOTTE 2024/2025 ACADEMIC PERIOD
OWNER: EMILLYCHARLOTTE
COPYRIGHT STATEMENT: ©2024 EMILLYCHARLOTTE. ALL RIGHTS RESERVED
FIRST PUBLISHED: SEPTEMBER 2024

What protects the DNA strand during replication? - Answer✔️✔️-Single-stranded binding

protein

Leading Strand - Answer✔️✔️-3' to 5' template

Lagging Strand - Answer✔️✔️-5' to 3' template

What DNA strand is being copied in transcription? - Answer✔️✔️-Antisense Strand

First step after newly formed mRNA is made? - Answer✔️✔️-Addition of poly(A) tail

Every time the chromosome is copied it would become shorter if not for what enzyme? -

Answer✔️✔️-Telomerase

What polymerase has repair activity? - Answer✔️✔️-Beta/Epsilon

What polymerase acts on leading strand? - Answer✔️✔️-Delta

What polymerase acts on lagging strand? - Answer✔️✔️-Alpha

Which polymerase is used for mitochondrial replication and repair? - Answer✔️✔️-

Gamma

What polymerase binds the 5' end to 3' end of dsDNA? - Answer✔️✔️-Topoisomerase

Which RNA polymerase is mainly for transcription? - Answer✔️✔️-RNA Pol II

Which polymerase is involved with mRNA synthesis? - Answer✔️✔️-RNA Pol II

What inactivates RNAses? - Answer✔️✔️-Diethyl pyrocarbonate (DEPC) or bake

glassware for 4 to 6 hours at 400C

What type of restriction enzyme recognizes palindromic sites? - Answer✔️✔️-Restriction

Enzyme Type II

,TITLE: EMILLYCHARLOTTE 2024/2025 ACADEMIC PERIOD
OWNER: EMILLYCHARLOTTE
COPYRIGHT STATEMENT: ©2024 EMILLYCHARLOTTE. ALL RIGHTS RESERVED
FIRST PUBLISHED: SEPTEMBER 2024

What type of restriction enzyme lacks methylation activity? - Answer✔️✔️-Restriction

Enzyme Type II

What is necessary to make cDNA? - Answer✔️✔️-Reverse transcriptase

Number of SNPs in a human genome? - Answer✔️✔️-11 billion (10^7)

How many genes (base pairs) are in a human haploid genome? - Answer✔️✔️-3 billion

base pairs

The polymerization of acrylamide requires: - Answer✔️✔️-Catalyst (APS or TEMED) or

Light activation

How does agarose gel pore size relate to concentration? - Answer✔️✔️-Inversely

proportional

What is denatured during a southern blot? - Answer✔️✔️-Hydrogen bonds (inter-strand

bonds)

Melting temperature formula - Answer✔️✔️-Tm = (4C x GC Pairs) + (2C x AT pairs)

Which is likely to interfere with fluorescent probe? - Answer✔️✔️-5' G

Proper GC content of primer - Answer✔️✔️-35-65%

Inhibitors of PCR - Answer✔️✔️-Hemoglobin, Heparin, Xylene, Phenol, Cyanol, and

Bromophenol Blue

Order to add to a PCR reaction: - Answer✔️✔️-Master mix > patient sample > positive

control > negative control

Pre Area - Answer✔️✔️-Positive Pressure

, TITLE: EMILLYCHARLOTTE 2024/2025 ACADEMIC PERIOD
OWNER: EMILLYCHARLOTTE
COPYRIGHT STATEMENT: ©2024 EMILLYCHARLOTTE. ALL RIGHTS RESERVED
FIRST PUBLISHED: SEPTEMBER 2024

Post Area - Answer✔️✔️-Negative Pressure

Which PCR requires two steps for specificity? - Answer✔️✔️-Nested PCR

Which microorganism is detected by branched DNA amplification? - Answer✔️✔️-DNA or

RNA

Which technique uses RNA, antibodies, and DNA? - Answer✔️✔️-Hybrid Capture Assay

Use of bisulfite treatment of DNA to determine its pattern of methylation - Answer✔️✔️-

Bisulfite DNA

What tests are isothermal? - Answer✔️✔️-NASBA, TMA, & SDA

Which leukemia is associated with FLT3 gene? - Answer✔️✔️-AML

T-cell gene rearrangement is seen in what malignancy? - Answer✔️✔️-Sezary Syndrome

Which translocation causes Acute Premyelocytic Leukemia? - Answer✔️✔️-t 15;17

Ewing Sarcoma - Answer✔️✔️-t 11;22

Synovial Sarcoma - Answer✔️✔️-t X;18

Follicular Lymphoma - Answer✔️✔️-t 14;18

What genes would be screened in a colorectal panel? - Answer✔️✔️-MSH2 & MLH1

What gene mutation causes a high risk of breast or ovarian cancer? - Answer✔️✔️-

BRCA1 and BRCA2

What does MSI test for? - Answer✔️✔️-Mutations in mismatch repair genes (MMR)

The benefits of buying summaries with Stuvia:

Guaranteed quality through customer reviews

Guaranteed quality through customer reviews

Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.

Quick and easy check-out

Quick and easy check-out

You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.

Focus on what matters

Focus on what matters

Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!

Frequently asked questions

What do I get when I buy this document?

You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.

Satisfaction guarantee: how does it work?

Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.

Who am I buying these notes from?

Stuvia is a marketplace, so you are not buying this document from us, but from seller EmillyCharlotte. Stuvia facilitates payment to the seller.

Will I be stuck with a subscription?

No, you only buy these notes for $12.49. You're not tied to anything after your purchase.

Can Stuvia be trusted?

4.6 stars on Google & Trustpilot (+1000 reviews)

81033 documents were sold in the last 30 days

Founded in 2010, the go-to place to buy study notes for 14 years now

Start selling
$12.49
  • (0)
  Add to cart