MCB 301 Exam Questions and Answers
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Pure Culture - Answer️️ -Population of cells consisting of one type of bacterium
derived from a single cell. a population of cells that are all clones and identical to each
other that originally came from a single cell. if you have a colony gro...
Differential Stain - Answer✔️✔️-Discriminates between different cell types. it usually
requires more than one dye.
The Gram Stain - Answer✔️✔️-The Gram Stain is a type of differential stain and it uses
the two dyes crystal violet and safranin.
Gram Stain Procedure - Answer✔️✔️-1. Heat Fix the cells to the slide
2. do the primary stain with crystal violet
3. fix the stain with iodine
3. decolorize with ethanol. the gram positive will hold onto the stain more strongly and
the gram negative don't hold onto the stain
4. stain with safranin which is the counter stain. the gram positive will stain purple and
the gram negative will be pink.
gram positive bacteria - Answer✔️✔️-cells stain purple based on cell wall structure.
gram negative - Answer✔️✔️-cells stain pink based on cell wall structure.
Gram positive cell wall - Answer✔️✔️-- there is a thick layer of peptidoglycan.
-it is composed of alternating N-acetylmuramic acid and n- acetylglucosamine residues.
-chains of peptidoglycan cross linked by peptides provide strength
-suface proteins NAM is linked to Teichoic acid and lipoteichoic acid is inserted in the
cytoplasmic membrane
-it prevents osmotic lysis.
-permeable to nutrients
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