MCB 301 final Exam Study Guide with Complete Solutions
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Course
MCB 301
Institution
MCB 301
MCB 301 final Exam Study Guide with
Complete Solutions
Which of the following statements about antibiotics is TRUE?
Antibiotics are used to treat fungal infections.
Some antibiotics work by binding the 40S ribosomal subunit.
Most of the earth's microorganisms have been tested for antibiotic pr...
The enzyme cleaves the beta-lactam ring of penicillin to inactivate it. (1 pt)
One of the assigned readings described genome mining and its use to discover
potentially new phosphonic acid products. Briefly describe how PCR was used in
genome mining described in the reading. - Answer✔️✔️-The genomes of various strains
of bacteria were each screened for a specific gene required for the synthesis of
phosphonic acid products. This was performed by PCR using DNA primers to
conserved regions of that gene. Generation of the amplification fragment by PCR
indicated that the bacterial strain possessed that gene and thus made a type of
phosphonic acid product. (2 pt)
Why do sulfa drugs (like sulfanilamide) have selective toxicity? Include in your answer
how sulfa drugs work. Note: The additional assigned readings may be helpful. -
Answer✔️✔️-Sulfa drugs inhibit the activity of an enzyme that makes a vitamin (i.e., folic
acid) required for the growth of both bacteria and humans. (1 pt)
However, bacteria make this enzyme, and humans do not (Note: humans therefore
require the vitamin preformed in their diet). Consequently, the drug affects the growth of
bacteria and not human cells (i.e., it has selective toxicity). (1 pt)
Comparison of 16S rRNA sequences from different bacterial species reveal regions that
are conserved and regions that are variable. How are these conserved regions and how
are these variable regions useful in the Antibiotic Producing Bacteria Exercise? -
Answer✔️✔️-Conserved regions located at the ends of the 16S rRNA gene can be used
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