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Portage Microbiology Exam 4 correct answers latest version
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Portage Microbiology Exam 4 correct answers latest version
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Portage Microbiology Exam 4 correctanswers latest version
True or False: Growth media is best suited for distinguishing between two similar species of bacteria
Answer: False. Growth media is designed to simply support (and not restrict) microbial growth
What are the requirements of a fastidious microbe? (short answer) Answer: A fastidious microbe is
an organism with complex growth requirements such that is absent it will not grow. Enriched medias
thus contain these specific and essential nutrients required for the growth of a particular subset of
microorganisms.
True or False: LB agar is classified as a selective, non-differential media Answer: False
What is agar used for in microbiology? (short answer) Answer: Agar is used to create a solid, smooth
surface on which microbes can grow
Alpha hemolysis (matching) Answer: Incomplete hemolytic activity
Beta hemolysis (matching) Answer: Complete hemolytic activity
Gamma hemolysis (matching) Answer: No hemolytic activity
Columbia CNA agar is most closely related to which media (multiple choice) Answer: CNA agar is
similar to VAP as it is also enriched with blood and allows for differentiation based on hemolytic patterns
True or False: Chocolate agar gets its brown color from cocoa to produce an enriched media Answer:
False
, In an attempt to detect the presence of the pathogenic strain of e coli O157:H7, a researcher spread
culture onto a MacConkey agar with failed results. What type of agar should they (correctly) try next?
Why? (short answer) Answer: The microbe should be plated on SMAC as it is specifically formulated
to detect O157:H7. Pathogenic e coli cannot ferment sorbitol while non-pathogenic e coli can ferment
both sorbitol and lactose. Therefore, colonies that ferment can be differentiated from non-fermenters.
What is the gram status of microbes growing on eosin methylene blue agar plates? (short answer)
Answer: Gram-negative
What is the process of spreading a bacterial culture onto a petri dish? (short answer) Answer:
Plating
Mannitol salt agar will turn what color in the presence of the pathogenic strain staphylococcus aureus?
(short answer) Answer: Yellow
In order to visual individual colonies of bacteria, would you culture your sample in a liquid media or on a
solid (agar) media? Why? (short answer) Answer: Solid media. The primary advantage is that cells
are held into place. When grown in a nutrient broth, bacterial cells can multiply but are free to move
around in solution. When grown on agar within a petri dish the fixed in such a way as to form colonies
True or False: the visualization of colonies on a petri dish represents bacterial cells that have often
multiplied a million times over Answer: True
True or False: the purpose of a quadrant streak expand a bacterial population Answer: False
To be considered a pure culture, the sample can be traced back to a single cell and _________ Answer:
The culture must also be free from external contaminants. Would not contain multiple bacterial species
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