Medical Microbiology VTPB 405 Exam #1 || Questions and 100% Verified Answers.
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Course
Medical Microbiology VTPB 405
Institution
Medical Microbiology VTPB 405
Extremeophiles correct answers too harsh for other life forms (ex. hot springs, high salt/acidity/alkalinity)
Ecosystem correct answers All living organisms, plus physical and chemical constituents of their environment.
Microbial ecology correct answers The study of microbes in their natural ...
Medical Microbiology VTPB 405 Exam #1 || Questions and
100% Verified Answers.
Extremeophiles correct answers too harsh for other life forms (ex. hot springs, high
salt/acidity/alkalinity)
Ecosystem correct answers All living organisms, plus physical and chemical constituents of their
environment.
Microbial ecology correct answers The study of microbes in their natural environment
- About 10 microbe cells per per human cell
Microorganisms correct answers Can be both beneficial and harmful, but most are beneficial
Gut microbiome correct answers Collection of microbes and their metabolites
- Digest complex carbohydrates in monogastrics
- Prevent colonization by pathogens, synthesizing vitamins, etc.
Negative Impacts Microorganisms have correct answers 1. Can cause food spoilage and food
borne diseases
2. Harvest, store, safety, prevention of spoilage influenced by microbes.
Magnification correct answers The ability to make an object larger
, Resolution correct answers The ability to distinguish two adjacent objects as distinct and
separate
- Limit for light microscope: 0.2um @ 1000x magnification
Bright Field Microscopy correct answers Specimens visualized because of differences in contrast
-Pigmented microbes add contrast
Light Microscopy (Phase-contrast) correct answers Improves image contrast of unstained, live
cells
- Resulting image: dark cells on a light background
Light Microscopy (Dark-Field) correct answers Light reaches the specimen from the sides
- Image appears light on a dark background
Differential interference contrast (DIC) Microscopy correct answers Uses a polarizer to create
two distinct beams of polarized light (light in a single plane).
- Gives structures such as nuclei, endospores, vacuoles, and inclusions a 3D appearance
Confocal Scanning Laser Microscopy (CSLM) correct answers Uses a computerized microscope
coupled with a laser source to generate a 3D image
- Has to have fluorescent dye to see
Electron Microscopy correct answers Use electrons instead of visible light to image cells and
structures.
-Much greater resolution than light microscopes
-Electromagnets function as lenses
-Operates in a vacuum
-2 different types
Transmission Electron Microscopes (TEM)(2D) correct answers Enables visualization of
structures at the molecular level
-Specimen must be very thin (20-60nm) and stained with high atomic weight substances that
scatter electrons well and improve contrast
- Negative staining allows direct observation of intact cells/components
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