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Exam (elaborations)

MLS ASCP Exam - MICRO With Questions And Accurate Answers

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  • September 16, 2024
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  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • MLS ASCP - MICRO
  • MLS ASCP - MICRO
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MLS ASCP Exam - MICRO With Questions And Accurate Answers



Disinfection, desiccation, filtration, fridge, heat, radiation, and Sterilization - Answer
Types of sterilization/inhibition techniques

denatures proteins

15 lbs ,121*C for 15 mins - Answer Moist Heat Sterilization

kills by oxidation

hot air oven 170*C for 2 hrs - Answer dry heat sterilization

140*C for 3 secs, NOT STERILIZATION - Answer pasturization sterilization

Damages cytoplasmic membrane and denatures protein - Answer Disinfectant: Phenol

Oxidizes (iodine and chlorine) - Answer Disinfectant: Halogens



Denatures proteins and dissolves lipids - Answer Disinfectant: Alcohols



penicillins and cephalosporins

cell wall synthesis - Answer B Lactams



Vancomycin

C Diff/MRSA

cell wall synthesis - Answer Glycoproteins



Gentamycin, tobramycin - Answer Aminoglycosides



-Tetracycline, doxycycline, minocycline

-Clinical use: Borrelia burgdorferi, M. pneumoniae. Drugs' ability to accumulate
intracellularly makes them very effective against Rickettsia and Chlamydia. Also used to
treat acne. - Answer Tetracyclines

,Meningitis (Haemophilus influenzae, Neisseria meningitidis, Streptococcus
pneumoniae) and Rocky Mountain spotted fever (Rickettsia rickettsii). - Answer
Chloramphenicol



erythromycin, clarithromycin, azithromycin - Answer Macrolides



Ciprofloxacin, norfloxacin



P aeruginosa - Answer Quinolines



UTI (Bactrim, sulfisoxazole)



Ulcerative colitis (Sulfasalazine)



folic acid metabolism - Answer Sulfa drugs



quinupristin/dalfopristin



vanco resistant E. Faecium - Answer Streptogramins



linezolid (Zyvox);



Used to treat methicillin resistant Staphylococcus aureus (MRSA) and vancomycin
resistant Enterococcus (VRE) - Answer Oxazolidinones



Aminoglycosides, cephalosporins, quinolones - Answer GNR Drugs

, RNA metabolism - Rifampin



killing harmful microbes without damaging the host - selective toxicity



Kills bacteria without host immune help - Bactericidal action



Inhibits organism growth - Bacteriostatic action



the minimum concentration of a substance necessary to prevent microbial growth -
Minimum inhibitory concentration MIC



minimum concentration of drug needed for killing of bacteria - Minimum bactericidal
concentration MBC



a more resistant group of beta-lactamases that hydrolyze extended spectrum
cephalosporins w/ an *oxyimino side chain*



(abx ex: cefotaxime, ceftriaxone, ceftazidime, aztreonam) - Answer ESBL (extended
spectrum beta-lactamase)



Test used to detect inducible clindamycin resistance in staph and β-streptococci.
Erythromycin disk in placed next to clindamycin disk on std. disk diffusion plate.
Flattening of the clind. zone between the two disks indicates inducible clindamycin
resistance. - Answer D Test



organisms that have become resistant to carbapenems, which are a class of antibiotics



K. Pneumo and E coli - Answer Carbapenem-resistant Enterobacteriaceae (CRE)



Swarming proteus zone around penicillin or ampicillin discus with B-Lactamase neg

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