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CMB2001 Questions and Answer | Latest Update 2024/2025

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Consensus sequences are described with IUPAC ambiguous nucleotide codes. What is a promoter? ~~> DNA sequence which RNA polymerase can bind to and transcribe the protein Prokaryotic promoters ~~> TATAAT -(10 region) 'Pribnow box' TTGACA - (-35 region) - two consensus sequences Pr...

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  • September 15, 2024
  • 12
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • CMB2001
  • CMB2001
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MASTER01
1 | P a g e | © copyright 2024/2025 | Grade A+




CMB2001 Questions and Answer |
Latest Update 2024/2025
Consensus sequences are described with IUPAC ambiguous nucleotide

codes.




What is a promoter?


✓ ~~> DNA sequence which RNA polymerase can bind to and

transcribe the protein




Prokaryotic promoters


✓ ~~> TATAAT -(10 region) 'Pribnow box'

TTGACA - (-35 region)


- two consensus sequences




Prokaryotic RNA polymerase


✓ ~~> 1 RNA Polymerase makes all 3 kinds of RNA

Composed of 5 subunits, four comprise the core enzyme




What is the core enzyme?



Master01 | September, 2024/2025 | Latest update

, 2 | P a g e | © copyright 2024/2025 | Grade A+


✓ ~~> Helps to initiate and synthesize RNA. 4 main polypeptides (2

alpha, 1 beta, 1 beta prime)




1 sigma bound only at initiation - forms holoenzyme




What is the purpose of the sigma subunit in RNA polymerase?


✓ ~~> Binds to RNA polymerase and allows core enzyme to transcribe

specific sites at the promoter




Start site in prokaryotic promoters


✓ ~~> TG/AT

+1


5-8bp




What is the span of the core/basal region of the eukaryotic promoter?


✓ ~~> -30 (TATA) (INR+1) to +30nt (DPE)




What elements are in the core region of the eukaryotic promoter?


✓ ~~> TATA box

Inr (initiator)


Master01 | September, 2024/2025 | Latest update

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