CMB2001 Questions and Answer | Latest Update 2024/2025
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Course
CMB2001
Institution
CMB2001
Consensus sequences are described with IUPAC ambiguous nucleotide
codes.
What is a promoter?
~~> DNA sequence which RNA polymerase can bind to and
transcribe the protein
Prokaryotic promoters
~~> TATAAT -(10 region) 'Pribnow box'
TTGACA - (-35 region)
- two consensus sequences
Pr...
✓ ~~> Helps to initiate and synthesize RNA. 4 main polypeptides (2
alpha, 1 beta, 1 beta prime)
1 sigma bound only at initiation - forms holoenzyme
What is the purpose of the sigma subunit in RNA polymerase?
✓ ~~> Binds to RNA polymerase and allows core enzyme to transcribe
specific sites at the promoter
Start site in prokaryotic promoters
✓ ~~> TG/AT
+1
5-8bp
What is the span of the core/basal region of the eukaryotic promoter?
✓ ~~> -30 (TATA) (INR+1) to +30nt (DPE)
What elements are in the core region of the eukaryotic promoter?
✓ ~~> TATA box
Inr (initiator)
Master01 | September, 2024/2025 | Latest update
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