MIP 300 Study Guide with Verified and Updated Solutions
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Course
MIP 300
Institution
MIP 300
MIP 300 Study Guide with Verified and
Updated Solutions
Bright Field Microscope - Answer️️ --produces a dark image against a brighter
background,
-Most common
-Must fix cells
-Color of stain=bacteria color
-100x requires oil
-Light is bet through objective
-Basic dyes binf to neg
-Aci...
MIP 300 Study Guide with Verified and
Updated Solutions
Bright Field Microscope - Answer✔️✔️--produces a dark image against a brighter
background,
-Most common
-Must fix cells
-Color of stain=bacteria color
-100x requires oil
-Light is bet through objective
-Basic dyes binf to neg
-Acidic dyes bind to pos
Dark Field Microscope - Answer✔️✔️--Background is dark, organism is light
-Light is refracted by organism
-in the field, dont have to heat fix or stain
ex.Syphilis
phase contrast microscope - Answer✔️✔️--Shades of grey to show contrast in cell
components
-slight differences in refractive indexes into light intensity
-Motility because no fixing or staining
fluorescence microscopy - Answer✔️✔️--Uses UV light to excite fluorescent molecules
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