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Exam (elaborations)

MIP 300 Study Guide with Verified and Updated Solutions

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  • MIP 300

MIP 300 Study Guide with Verified and Updated Solutions Bright Field Microscope - Answer️️ --produces a dark image against a brighter background, -Most common -Must fix cells -Color of stain=bacteria color -100x requires oil -Light is bet through objective -Basic dyes binf to neg -Aci...

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  • September 13, 2024
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  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • MIP 300
  • MIP 300
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, ©SOPHIABENNETT EXAM SOLUTIONS Saturday, September 7, 2024 6:58 PM



MIP 300 Study Guide with Verified and
Updated Solutions


Bright Field Microscope - Answer✔️✔️--produces a dark image against a brighter

background,

-Most common

-Must fix cells

-Color of stain=bacteria color

-100x requires oil

-Light is bet through objective

-Basic dyes binf to neg

-Acidic dyes bind to pos

Dark Field Microscope - Answer✔️✔️--Background is dark, organism is light

-Light is refracted by organism

-in the field, dont have to heat fix or stain

ex.Syphilis

phase contrast microscope - Answer✔️✔️--Shades of grey to show contrast in cell

components

-slight differences in refractive indexes into light intensity

-Motility because no fixing or staining

fluorescence microscopy - Answer✔️✔️--Uses UV light to excite fluorescent molecules


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