Uvic Biol 438 Final Exam Questions and Correct Answers
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Course
BIO 438
Institution
BIO 438
Oxygenic photosynthesis -found in cyanobacteria -uses H2O as proton donor -Produces O2 -2 photosystems -linear flow of electrons -uses Calvin cycle -uses Chl a
Anoxygenic photosynthesis -uses H2S as proton donor -produces S -green sulphur bacteria use reverse cycle to incorporate CO2 rather than p...
Uvic Biol 438 Final Exam Questions and
Correct Answers
Oxygenic photosynthesis ✅-found in cyanobacteria
-uses H2O as proton donor
-Produces O2
-2 photosystems
-linear flow of electrons
-uses Calvin cycle
-uses Chl a
Anoxygenic photosynthesis ✅-uses H2S as proton donor
-produces S
-green sulphur bacteria use reverse cycle to incorporate CO2 rather than produce it
-green non-sulphur bacteria use the hydroxypropionate pathway (ancestral pathway)
Photosystem I ✅-similar to Chlorobi (Green S. bacteria) and Firmicutes
(Heliobacterium)
-has ferrodoxin
Photosystem II ✅-similar to Proteobacteria and green non-sulphur bacteria
(Chloroflexi)
-has reverse electron flow
Photosynthesis genes ✅PufM and PufL
-PufM tells you photosynthesis is present in organism and what type of photosynthesis it
is.
Methods to measure photosynthesis ✅-epifluorescence microscopy or determination
of pigment (Chlorophyl a or bacteriochlorophyl).
-Map O2 profile. Photosynthesis appears as peak in O2.
metabolic inhibitor of photosynthesis ✅-DCMU, blocks e- flow b/t PS I and PS II.
Affects oxygenic photosynthesis but not anoxygenic photosynthesis (negative control).
Lake Mahoney ✅-high sulphur content. Mountains near by have high [ ] of sulphur.
-chromatium and lamprocystis purpureus found here
-
Heliophilum Fasciatum ✅-found in rice patties
-important b/c they can fix nitrogen
Lake Csiso ✅-high amount of H2S
, -bacteria found about 2m down (gradient from O2 to H2S at this depth)
-7m down is where Lamprocystis purpurea is found
Winogradsky column ✅-glass filled with dirt and eggs shells/ paper (C source) and
topped with water incubated. Colour of water changes over time.
F.I.S.H ✅-Fluorescent In-Situ Hybridization
- can locate bacteria in soil and determine conditions of soil where bacteria located.
Mt Doug photosynthetic bacteria ✅
Nanopore sequencing ✅Unwind DNA and pass single strand through a small
nanopore and measure the change in current (current specific to diff. nucleotide bases).
What important photosynthetic bacteria was found in all soil samples?
✅Rhodopseudomonas
Respiration ✅Conversion of sugars to CO2
-glycolysis, tricarboxylic acid cycle and oxidative phosphorylation.
-important for degradation of organic matter
Citric acid cycle ✅Produces NADH and ATP
-coenzyme a important for this cycle
Coenzyme A ✅-modified adenine
-has an HS group which is used to bind acetyl compound to it
Xenobiotics ✅-have bonds which slow down chemical degredation
-recalcitrant (not easily broken down)
-most related to some kind of derivative of petroleum.
Biodegradation ✅Transformation of compound but not complete degradation.
-DDT used in first biodegradation studies
Minieralization ✅Complete degradation and recycling of an organic molecule into its
mineral constituents.
Organisms mineralizing aliphatic hydrocarbons ✅-P. putida, A. borkumensis, B.
cepacia, and R. erythropolis
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