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GMS6504 chromatography MS Exam Questions And Correct Answers

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GMS6504 chromatography MS Exam Questions And Correct Answers...

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  • September 12, 2024
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  • 2024/2025
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  • gms6504 chromatography ms
  • GMS6504 chromatography MS
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GMS6504 chromatography MS Exam Questions
And Correct Answers


partitioning

2

chromatographic efficiency

3

classes of mass spectrometers

4

solvent strength

Terms in this set (27)

chromatography

separates compounds based on differential preference to bind to TWO PHASES

mobile phase

stationary phase

partitioning

upper phase

lower phase

basis of separation

adsorption

partition

ion-exchange chromatography

size-exclusion chromatography

electrophoresis = smaller particles move faster ?

column chromatography

, column used

solid for stationary phase

liquid for mobile phase

gravity pulls mixture down = can separate

chromatographic efficiency

ability to separate one compound from another

variables that affect resolution

-d = how far apart the peaks are

-the greater the d = the better the resolution

-w = width of the peaks = the lower w is (narrower) the better the resolution is

ways to improve resolution

-moving the peaks further apart

-make them skinnier

phase polarity

-polarity of analyte, stationary phase, and mobile phase determines retention

-reverse phase is most common = non-polar stationary phase and polar mobile phase

polarity

-intermolecular forces btwn mobile phase and sample, and stationary phase and sample
govern retention of solutes gel permeation chromatography -AKA size exclusion
chromatography -separates based on molecular size -stationary phase filled with pores
-large molecules are too large to fit so are excluded = pass through quickly -small
molecules permeate the stationary phase = pass through slowly mechanisms of
chromatography adsorption: -most common for small molecules -two types of
adsorbents = nonpolar and polar -most common are silica and alumina

ion exchange chromatography

-mobile phase is NEUTRAL

-stationary phase is CHARGED

-anion exchange = A- + R+B- --> B- + R+A-

-cation exchange = A+ + R-B+ --> B+ + R-A+

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