rifai tietz fundamentals of clinical chemistry and
8 th edition tietz fundamentals of clinical chemis
tietz fundamentals of clinical chemistry and molec
Connected book
Book Title:
Author(s):
Edition:
ISBN:
Edition:
More summaries for
TEST BANK For Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics, 8th Edition by Nader Rifai, Verified Chapters 1 - 49, Complete Newest Version
All for this textbook (2)
Written for
Tietz Fundamentals of Clinical Chemistry and Molec
All documents for this subject (16)
Seller
Follow
kushboopatel6867
Reviews received
Content preview
TEST BANK For Tietz Fundamentals of Clinical Chemistry and
Molecular Diagnostics, 8th Edition by Nader Rifai,
amino acid - ANSWER:an organic compound that contains an amino group (-NH2)
and a carboxyl group(-COOH)
List the essential amino acids - ANSWER:Phenylalanine
Valine
Threonine
Tryptophan
Isoleucine
Methionine
Arginine
Leucine
Lysine
Primary aminoaciduria - ANSWER:inherited enzyme defect (inborn error of
metabolism) or genetic diseases that leads to the absence of a single amino acid
secondary aminoaciduria - ANSWER:due to disease or damage of an organ such as
liver or generalized renal tubular dysfunction or protein-energy malnutrition
Methods used to analyze AA - ANSWER:1. ion exchange chromatography/ninhydrin
system
2. LC-MS/MS
3. reversed-phase HPLC
4. capillary electrophoresis (CE)
5. GC
peptide bond - ANSWER:The covalent bond or link formed by a condensation
reaction between the amino group or one amino acid with the carboxyl group of
another to form an amide linkage
Quantitative analysis of proteins includes - ANSWER:1. physical methods
2. activity measurements
3. immunoassays
Qualitative analysis of proteins includes - ANSWER:1. electrophoresis
2. chromatography
3. genetic analysis
4. functional assays
5. mass spectrometry
Methods for measuring TP in fluids - ANSWER:1. Kjeldahl
2. biuret
Kjeldahl method - ANSWER:sample digested with acid to convert nitrogen in protein
to ammonium ion that is measured; reproducible, but inconvenient, time-
consuming, impractical
Biuret Method - ANSWER:Cu2+ complexes with peptide bonds in alkaline conditions
causing a shift in color from blue to violet; moderate sensitivity; slightly affected by
icterus and lipemia
Direct Photometric Methods - ANSWER:absorbance of ultraviolet light at 200-225
nm (tyrosine and tryptophan) and 270-290 nm (peptide bonds)
Dye Binding Method - ANSWER:shifts in absorbance spectra of dyes when bound to
proteins; good sensitivity and low interference from small compounds
Pyrogallol Red - ANSWER:used for the analysis of protein in dilute fluids such as urine
and CSF
Refractometry - ANSWER:measurement of of refractive index; not normally used for
TP determination
Turbidimetric method - ANSWER:measures changes in absorbance caused by
aggregated protein
Nephelometric method - ANSWER:measures light reflected at an angle by
aggregated protein
Common reagents used in tubidmetric methods - ANSWER:1. tricholoroacetic acid
2. SSA
3. SSA with sodium sulfate
4. benzethonium chloride
5. benzalkonium salts
Turbidimetric and nephelometric assay can be - ANSWER:end point or kinetic
reactions
Three dyes used to stain protein in serum protein electrophoresis - ANSWER:1.
amido black
2. Ponceau S
3. Coomassie brilliant blue
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller kushboopatel6867. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $17.99. You're not tied to anything after your purchase.
