URINALYSIS AND BODY FLUIDS Questions and Correct Answers | Latest Update
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Course
Urinalysis and body fluids
Institution
Urinalysis And Body Fluids
Lecithin: sphingomyelin ratio (L:S Ratio)- what is the purpose, and the L:S ratio.
-:- Purpose= Assessment of *fetal lung maturity*
Method= Thin layer *chromatography*.
Comment= L:S ratio *>2.0 signifies maturity*
Foam stability index (shake test)
-:- Purpose= assessment of *fetal lung m...
URINALYSIS AND BODY FLUIDS
Questions and Correct Answers | Latest
Update
Lecithin: sphingomyelin ratio (L:S Ratio)- what is the purpose, and the L:S ratio.
✓ -:- Purpose= Assessment of *fetal lung maturity*
Method= Thin layer *chromatography*.
Comment= L:S ratio *>2.0 signifies maturity*
Foam stability index (shake test)
✓ -:- Purpose= assessment of *fetal lung maturity*.
Method= Shake with *95% ethanol*.
Comment= Foam stability index= tube with *the highest ethanol* volume fraction that will
result in a stable ring of *bubbles at the meniscus* after shaking. An index of *0.48* is
comparable with an *L:S ratio of 2.0*
Amniotic fluid bilirubin
✓ -:- Purpose=Diagnosis of *hemolytic disease of the newborn*.
Method= *Direct spectrophotometric* scan from *365-550* nm.
Comment= Bilirubin has peak absorbance at *450*nm. A baseline is drawn connecting the
365 and 550 nm points. The change of a 450 is to compared to gestational age to determine
severity of disease. *The specimen must be protected from light, blood and medium
interfere*.
Source of error:
a. False-positive: highly *buffered or alkaline urine*, prolonged *dipping*.
b. False-negative: *Protein* other than *albumin*.
Comment=
- Buffered to maintain *pH 3*.
- *Most sensitive to albumin*.
-*Blood, WBCs, bacteria* can cause *positive reaction*.
- Orthostatic proteinuria: a *benign condition* in which protein is *negative in first AM*
specimen and *positive after standing*.
Source of error:
a. False-positive: contamination with (2)
b. False-negative: high levels of (2)
Comment= what is specific for, and more sensitive than-----, how long after eating?
✓ -:- Normal= Negative.
Principle= Glucose oxidase/ Peroxidase.
Significance= *Diabetes mellitus*.
Source of error:
a. False-positive: contamination with *peroxide or oxidizing detergents (bleach)*
b. False-negative: high levels of *ascorbic acid, glycolysis*.
Comment=
- Specific for glucose.
- *More sensitive and specific than copper reduction test*.
- For diabetic monitoring specimen collected *2 hours after eating* is referred.
- Normal renal threshold=*160-180 mg/dl*
Ketone
Normal=
Principle=
Significance= (5)
Source of error:
a. Decrease in
Comment= Most sensitive to
4|P a g e | G r a d eA + | 2 0 2 0 2 5
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