Molecular Biology Of The Cell Exam 3
Questions And Answers Latest Update
2024/2025
1. Which of the following is NOT observed in chromatin in eukaryotic cells?
A) The presence of many other proteins beside histones.
B) DNA being looped twice around each histone octomer.
C) The condensation or packing state being set (not changable) once it is established.
D) Adjacent nucleosomes connecting in a helical manner to form a thicker fiber.
E) Loops of 30 nm fibers extending out from a protein scaffold. Answer: C) The condensation or
packing state being set (not changable) once it is established.
2. You are in the middle of the process of determining the genome sequence of an
organism by using the shotgun approach. You have obtained a sequencing read from
the sequencing of an insert in a single plasmid. What is the next step?
A) Figure out from which artificial chromosome the piece comes from by Southern
blot
B) Nothing, can't do anything until you have enough sequencing reads to cover the
genome 20 times
C) Use a computer to see if it has overlaps with any other sequencing reads previously
obtained
D) Figure out from which chromosome the sequence comes from by in situ
hybridization
E) Use a computer to search for similar sequences in other organisms using Blast Answer: C) Use a
computer to see if it has overlaps with any other sequencing reads previously
, obtained
3. Which of the following is a difference between the genome of the bacterium E. coli and
the genome of the humans?
A) The size of the E. coli genome is larger.
B) The E. coli genome has more repetitive DNA sequence elements
C) The E. coli genome contained more genes.
D) The E. coli gene more frequently contained introns.
E) The E. coli genome has a higher percentage of sequences that code for proteins Answer: E) The E.
coli genome has a higher percentage of sequences that code for proteins
4. Heterochromatin consists of
A) 10-nm chromatin fibers.
B) highly condensed, transcriptionally inactive chromatin.
C) DNA associated with nucleosomes.
D) decondensed, transcriptionally active chromatin Answer: B) highly condensed, transcriptionally
inactive chromatin.
5. You are analyzing the sequence of a chromosome from the genome of the chimpanzee
and have found a region on the chromosome where there is 300 copies of the same 10
base long sequence i.e. ...AGTTCAATCGAGTTCAATCG... . What type of genomic
element have you found?
A) A DNA transposon
B) A LINE
C) A SINE
D) A gene family
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