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Exam (elaborations)

OSMT Histology Exam Questions and Answers All Correct

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  • OSMT Histology

OSMT Histology Exam Questions and Answers All Correct What are 3 transport medias they use for tissues - Answer-1) Saline- for unfixed STAT 2) Michaels transport medium 3) fixative What is michaels transport medium - Answer-it is commercial saline-like preservatives for unfixed tissue to ...

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  • September 5, 2024
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  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • OSMT Histology
  • OSMT Histology
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OSMT Histology Exam Questions
and Answers All Correct

What are 3 transport medias they use for tissues - Answer-1) Saline- for unfixed STAT
2) Michaels transport medium
3) fixative

What is michaels transport medium - Answer-it is commercial saline-like preservatives
for unfixed tissue to allow for transportation or delay of fixation

What is the best transport medium to use for tissues - Answer-fixative

When transporting wet tissue specimens what type of packing is it and what is on the
label - Answer--in a plastic leak proof container with screw cap lids
-patient info
-whmis labels
-clearly labelled with shipping and return address

When transporting slides what packaging are they sent in - Answer-cardboard or plastic
mailing envelopes

What are specimens called in order to remove it , it has to be done by surgery - Answer-
irretrievable specimen

What is grossing - Answer-looking at the sample in greater detail

-examining and describing

What are block sections - Answer-sections that are cut from the tissue that are being
examined for interest

What is the thickness of an ideal block section - Answer-3-4 mm thick

Where are block sections placed into - Answer-cassettes

What are some specimens (objects) that are not processed - Answer-bullets, implants,
impaled objects

What is fixation - Answer-to examine tissue thoroughly to harden and preserve

What is the aim of fixation - Answer-to preserve the tissue in a lifelike manner

, What the key of fixation - Answer-stabilize proteins

What is the goal of fixation - Answer-to prevent putrefaction which is the destruction of
cells by bacterial pathogens or normal flora

what are the effects of fixation (4) - Answer-1) hardens tissue
2) makes tissue easier to work with
3) protects tissue from hard chemicals
4) enhances staining by making the tissue more receptive to dyes

what happens if the tissue over hardens - Answer-the tissue shrinks and becomes brittle

what factor influence fixation - Answer-higher temps= faster fixation

what is the ideal temp for fixation - Answer-usually done at RT (22)

how can agitation help with fixation - Answer-leads to faster and better fixation
leads to better penetration of fixative

how much fixative should you have ? - Answer-20x the volume of tissue

what happens if the tissue is to dense - Answer-longer to fix

Are CT capsules difficult to penetrate - Answer-yes

how do you fix hollow organs - Answer-fill or pin flat

what size should an ideal tissue block be - Answer-the size of a nickel

what are the 3 kinds of fixative categories - Answer-physical , simple and compound

what are physical fixatives - Answer-heat, microwave, freeze dry

what are simple fixatives - Answer-use once chemical , called primary fixative

what are compound fixatives - Answer-2 or more fixatives
-combination of one or more

what is the workflow for histology (7) - Answer-1) specimen collection
2) grossing
3) fixation
4) processing
5) embedding
6) microtomy
7) routine staining

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