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Protein Techniques (MCBM Exam 1) Questions with 100% Correct Answers
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Protein Techniques (MCBM Exam 1) Questions with 100% Correct Answers
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1 of 35Term
Prosthetic Group in metalloproteins
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metal flavin nucleotides
salt carbohydrates
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2 of 35
Term
centrifugation separates molecules based on their differences in ____
or _____
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volume or mass mass or density
sparsity or disperse charge
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3 of 35
Term
Crime Scene
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-same technique as ELISA but has to do with CELLS instead of proteins
, -use a chemical reagent (like NINHYDRIN) which binds to FREE AMINE
GROUPS on the protein
-this is how they look for fingerprints/blood spatter in crime scenes
-these chemical reagents (like Ninhydrin) absorb light
1. Put sample into tube
2. Centrifuge (spin it around)
3. Larger particles will pellet on the bottom of the tube
4. Smaller particles will stay in the liquid (supernatant)
-the more protein you have, the more absorbance you have (due to the aromatic
aa) the less transmission you will have
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4 of 35
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Mass Spectrometry separates proteins based on
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mass-to-charge ratio (m/z) charge
binding specificities mass or density
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5 of 35
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