BCHM 270 Exam Questions| Already
Answered| GRADED A+
DNA - ANSWER-Nucleotides: Composed of ATGC
Double stranded
Sugar: Deoxyribose
Overall polarity: Less polar
RNA - ANSWER-Nucleotides: Composed of AUGC
Single stranded
Sugar: Ribose
Overal polarity: More polar
Silent mutation - ANSWER-Does not change the amino acid
Missense mutation - ANSWER-Changes the amino acid
Nonsense mutation - ANSWER-Premature stop codon
Frameshift mutation - ANSWER-Insertion or deletion of nucleotide
Southern blot - ANSWER-DNA; detect large changes in DNA, point mutations that create/destroy
restriction sites
Northern blot - ANSWER-RNA; measure presence and relative size of mRNA
Western plot - ANSWER-Protein; measure presence and relative size of protein
, Allele specific oligonucleotides (ASO) / probing - ANSWER-DNA; detect single nucleotide differences in
DNA
Genotyping microarray - ANSWER-DNA; measure many genotypes at once (You are looking at mRNA
transcripts between different samples)
Enzyme-linked immunosorbent assay (ELISA) - ANSWER-Protein; detect proteins (antigens) or antibodies
(quantitatively determining protein amount)
Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) - ANSWER-Protein; separate
proteins by size
Polymerase chain reaction (PCR) - ANSWER-DNA; amplify sections of DNA using primers
Cloning - ANSWER-DNA; insert DNA sequence into a vector, and introduce to a cell (plasmid DNA is
manipulated)
Restriction endonuclease - ANSWER-DNA; cleaves DNA at defined palindromic sequences
CRISPR-Cas9 - ANSWER-DNA; edit genomic DNA in living cells
Alanine - ANSWER-Ala; A
Nonpolar
Glycine - ANSWER-Gly; G
Nonpolar
Leucine - ANSWER-Leu; L
Nonpolar
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