Exam (elaborations)
CGI EXAM #2 QUESTIONS WITH CORRECT ANSWERS
- Course
- Institution
CGI EXAM #2 QUESTIONS WITH CORRECT ANSWERS
[Show more]
Content preview
CGI EXAM #2 QUESTIONS WITHCORRECT ANSWERS
Central Dogma of Molecular Biology - Answer-DNA (replication) --> (transcription) RNA
--> (translation) Protein
DNA synthesized in the - Answer-5' to 3' direction
Replication Fork - Answer-Draw
DNA is synthesized in the 5' to 3' direction because the DNA polymerase must
recognize a - Answer-3'-OH
helicase - Answer-the enzyme that assists in unwinding the tightly coiled DNA
DNA polymerase I - Answer-the enzyme that removes the RNA primer sequences
PRIASE - Answer-is an RNA polymerase
ligase - Answer-seals the nicks in the sugar-phosphate DNA backbone to complete
lagging strand synthesis
DNA polymerase III - Answer-the enzyme that actually synthesizes the newly formed
leading strand, has proofreading ability
RNA polymerase II - Answer-creates the mRNA strand
telomerase - Answer-a single-stranded DNA polymerase that asists in replication ends
of eukaryotic chromosomes
tomoisomerase - Answer-the enzyme that relieves DNA tension by forming nicks in the
phosphodiester linked backbone
traditional cycle of PCR temperature and steps - Answer-94 C- denaturation, 55 C-
annealing, 72 C- elongation
denaturation - Answer-breaks H-bonds of DNA strands
annealing - Answer-short primers bind to ends of target sequences
elongation - Answer-Taq polymerase adds dNTPs to template strands amplifying the
target gene
, prokayotes have _ factors that help guide the RNA polymerase to the promoter region -
Answer-sigma
RNA polymerase synthesizes RNA in the 5' to 3' direction based on a - Answer-
template DNA sequence
in eukaryotes, _ transcribes all protein-coding genes - Answer-RNA polymerase II
gene expression - Answer-creating an mRNA from a gene
differential gene expression results in - Answer-different cell types
transcription begins at the - Answer-transcription start site
3 main types of RNA in cells - Answer-messenger RNA, ribosomal RNA, and transfer
RNA
messenger RNA - Answer-made from protein-coding genes; is translated into protein in
the cytoplasm
ribosomal RNA - Answer-form the ribosome (an enzyme that catalyzes translation)
along with some proteins
transfer RNA - Answer-bring specific amino acids to the ribosome during translation
DNA coding strand and _ have the same sequence - Answer-mRNA
Dna template strand - Answer-used to build the mRNA
prokaryotic transcription players - Answer-1. gene (promoter region with -35 and -10
elements), transcription start site, terminator region, 5' and 3' untranslated regions, 2.
sigma factor, 3. RNA polymerase
gene runs from _ to _ - Answer-promoter region to terminator region
prokaryotic transcription initiation - Answer-sigma factor with RNA polymerase bind to
promoter site
prokaryotic transcription elongation - Answer-mRNA synthesis begins, sigma factor
released, RNA polymerase elongates mRNA
prokaryotic transcription termination - Answer-RNA polymerase falls off DNA at
terminator sequence
the transcription start site is not the same as the - Answer-translation start site
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller biggdreamer. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $12.49. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
81989 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now